Western Blot (WB)
(Western blot analysis of MED28 in human brain tissue lysate with MED28 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.)
Rabbit anti-Human, Mouse MED28 Polyclonal Antibody | anti-MED28 antibody
MED28 Antibody
Gene Names
MED28; EG1; magicin; 1500003D12Rik
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity chromatography purified via peptide column
Synonyms
MED28; Polyclonal Antibody; MED28 Antibody; Mediator of RNA polymerase II transcription subunit 28; magicin; EG1; anti-MED28 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Affinity chromatography purified via peptide column
Form/Format
Supplied in PBS containing 0.02% sodium azide.
Sequence Length
178
Applicable Applications for anti-MED28 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Immunogen Type
Peptide
Immunogen Description
Raised against a 17 amino acid peptide from near the center of human MED28.
Target Name
MED28
Preparation and Storage
Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of MED28 in human brain tissue lysate with MED28 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.)
Western Blot (WB)
(Western blot analysis of MED28 in human brain tissue lysate with MED28 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.)
Immunohistochemistry (IHC)
(Immunohistochemistry of MED28 in human brain tissue with MED28 antibody at 2.5 ug/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of MED28 in human brain tissue with MED28 antibody at 2.5 ug/mL.)
Related Product Information for anti-MED28 antibody
The mediator complex is a multi-protein transcriptional co-activator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factor. One of the proteins in this complex is MED28, also known as Magicin. MED28 is expressed in many cell lines and tissues. It has been shown that a down-regulation of MED28 expression in NIH3T3 cells results in a significant induction of several genes associated with smooth muscle cell differentiation and conversely its over-expression represses expression of SMC genes. MED28 can also form a ternary complex with Grb2 and Merlin, the neurofibromatosis 2 tumor suppressor protein, indicating that MED28 may play a role in Merlin's tumor suppressive activity. MED28 has also been recently identified as an HIV dependency factor (HDF), suggesting that MED28 may be an important drug target in HIV treatment. At least two isoforms of MED28 are known to exist.
Product Categories/Family for anti-MED28 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,520 Da
NCBI Official Full Name
mediator of RNA polymerase II transcription subunit 28
NCBI Official Synonym Full Names
mediator complex subunit 28
NCBI Official Symbol
MED28
NCBI Official Synonym Symbols
EG1; magicin; 1500003D12Rik
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 28
UniProt Protein Name
Mediator of RNA polymerase II transcription subunit 28
Protein Family
UniProt Gene Name
MED28
UniProt Synonym Gene Names
EG1; Magicin
UniProt Entry Name
MED28_HUMAN
Uniprot Description
MED28: a component of the mediator complex that can either repress or activate transcription. Mediator complexes are essential for basal and regulated expression of nearly all RNA polymerase II-dependent genes. They may act as a bridge, conveying regulatory information from enhancers and other control elements to the promoter. May be part of a complex containing NF2/Merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways. Up-regulated by endothelial cells when exposed to tumor conditioned media.
Protein type: Transcription factor
Chromosomal Location of Human Ortholog: 4p16
Cellular Component: cortical actin cytoskeleton; membrane; Srb-mediator complex
Molecular Function: protein binding; actin binding
Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; stem cell maintenance; negative regulation of smooth muscle cell differentiation
Research Articles on MED28
Similar Products
Product Notes
The MED28 med28 (Catalog #AAA9406598) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MED28 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MED28 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the MED28 med28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MED28, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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