Rabbit NGFR Polyclonal Antibody | anti-NGFR antibody

NGFR (Nerve Growth Factor Receptor, CD271, Gp80-LNGFR, TNFRSF16, p75(NTR), p75NTR) (FITC)

Cat. Num. AAA6429095

• Gene Names: NGFR; CD271; p75NTR; TNFRSF16; p75(NTR); Gp80-LNGFR
• Reactivity: Human, Mouse, Rat
• Applications: Immunocytochemistry, Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Purified by affinity chromatography.
• Synonyms: NGFR; Polyclonal Antibody; NGFR (Nerve Growth Factor Receptor; CD271; Gp80-LNGFR; TNFRSF16; p75(NTR); p75NTR) (FITC); anti-NGFR antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes NGFR. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-NGFR antibody: Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: ICC: 1:50-1:200; WB: 1:500-1:2000; IHC: 1:50-1:200; IP: 1:20-150; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to human NGFR.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using NGFR antibody.)

Product Categories/Family for anti-NGFR antibody

Antibodies; Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 4505393
• NCBI GeneID: 4804
• NCBI Accession #: NP_002498.1
• NCBI GenBank Nucleotide #: NM_002507.3
• UniProt Accession #: P08138; B2R961
• Molecular Weight: 45,183 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 16
• NCBI Official Synonym Full Names: nerve growth factor receptor
• NCBI Official Symbol: NGFR
• NCBI Official Synonym Symbols: CD271; p75NTR; TNFRSF16; p75(NTR); Gp80-LNGFR
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 16; p75 ICD; NGF receptor; TNFR superfamily, member 16; low affinity nerve growth factor receptor; low affinity neurotrophin receptor p75NTR; low-affinity nerve growth factor receptor
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 16
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: NGFR
• UniProt Synonym Gene Names: TNFRSF16; NGF receptor
• UniProt Entry Name: TNR16_HUMAN

NCBI Description

Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. [provided by RefSeq, Jul 2008]

Uniprot Description

NGFR: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Homodimer; disulfide-linked. Interacts with p75NTR- associated cell death executor. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1 and NGFRAP1/BEX3. Interacts with KIDINS220 and NTRK1. Can form a ternary complex with NTRK1 and KIDINS220 and this complex is affected by the expression levels of KIDINS220. An increase in KIDINS220 expression leads to a decreased association of NGFR and NTRK1. Interacts with NTRK2; may regulate the ligand specificity of the NTRK2 receptor. Interacts with LINGO1.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 17q21-q22

Cellular Component: nucleoplasm; cell surface; integral to plasma membrane; extracellular region; plasma membrane; cytosol; endosome

Molecular Function: signal transducer activity; protein binding; transmembrane receptor activity; ubiquitin protein ligase binding; death receptor activity; nerve growth factor binding; receptor activity; Rab GTPase binding

Biological Process: detection of temperature stimulus; axon guidance; hair follicle morphogenesis; membrane protein intracellular domain proteolysis; central nervous system development; nerve growth factor receptor signaling pathway; negative regulation of fibroblast growth factor receptor signaling pathway; positive regulation of apoptosis; positive regulation of odontogenesis of dentine-containing teeth; regulation of caspase activity; glucose homeostasis; regulation of axonogenesis; negative regulation of hair follicle development; negative regulation of cell cycle; intracellular protein transport; positive regulation of fibroblast proliferation; negative regulation of axonogenesis; positive regulation of axonogenesis; nerve development; circadian regulation of gene expression; negative regulation of apoptosis