Rabbit anti-Human Me2-H4 (K20) Polyclonal Antibody | anti-HIST1H4A antibody

Me2-H4 (K20) Antibody

Cat. Num. AAA9208772

• Gene Names: HIST2H4B; H4/o
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot, Dot Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Me2-H4 (K20); Polyclonal Antibody; Me2-H4 (K20) Antibody; Histone H4; HIST1H4A; H4/A; H4FA; anti-HIST1H4A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This H4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from human H4.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.264 (varies by lot)
• Sequence Positions: 1-30
• Sequence Length: 103

• Applicable Applications for anti-HIST1H4A antibody: ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB), Dot Blot (DB)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of lysate from K562 cell line, using H4 Antibody (K20). MBS9208772 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)

Dot Blot (DB)

(Dot blot analysis of anti-hH4-K20(Methyl 2) methylation-specific Pab on nitrocellulose membrane. 50ng of methylation-peptide or Non methylation-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PSMA4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(H4 Antibody (K20 [Me2]) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-HIST1H4A antibody

Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones.

NCBI and Uniprot Product Information

• NCBI GI #: 77539758
• NCBI GeneID: 554313
• NCBI Accession #: NP_001029249.1; NP_003486.1; NP_003529.1; NP_003530.1; NP_003531.1; NP_003532.1; NP_003533.1; NP_003534.1; NP_003535.1; NP_003536.1; NP_003537.1; NP_003539.1; NP_068803.1; NP_778224.1
• NCBI GenBank Nucleotide #: NM_001034077.4
• UniProt Accession #: P62805; P02304; P02305; Q6DRA9; Q6FGB8; Q6NWP7; A2VCL0
• Molecular Weight: 11367
• NCBI Official Full Name: histone H4
• NCBI Official Synonym Full Names: histone cluster 2, H4b
• NCBI Official Symbol: HIST2H4B
• NCBI Official Synonym Symbols: H4/o
• NCBI Protein Information: histone H4
• UniProt Protein Name: Histone H4
• UniProt Gene Name: HIST1H4A
• UniProt Synonym Gene Names: H4/A; H4FA
• UniProt Entry Name: H4_HUMAN

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. [provided by RefSeq, Jul 2008]

Uniprot Description

H4: histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The gene encoding H4 is intronless.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6p22.2

Cellular Component: nucleoplasm; nuclear chromosome; protein complex; membrane; extracellular region; nucleosome; nucleus

Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity

Biological Process: chromatin silencing at rDNA; establishment and/or maintenance of chromatin architecture; DNA replication-dependent nucleosome assembly; regulation of gene expression, epigenetic; negative regulation of megakaryocyte differentiation; nucleosome assembly; DNA replication-independent nucleosome assembly at centromere; DNA replication-independent nucleosome assembly; negative regulation of gene expression, epigenetic; protein heterotetramerization; gene expression; mitotic cell cycle; telomere maintenance; DNA methylation on cytosine