Human Serine/Arginine Rich Splicing Factor 2 (SRSF2) ELISA Kit | SRSF2 elisa kit

Human Serine/Arginine Rich Splicing Factor 2 (SRSF2) ELISA Kit

Cat. Num. AAA2705688

• Gene Names: SRSF2; SC35; PR264; SC-35; SFRS2; SFRS2A; SRp30b
• Reactivity: Human
• Synonyms: Serine/Arginine Rich Splicing Factor 2 (SRSF2); Human Serine/Arginine Rich Splicing Factor 2 (SRSF2) ELISA Kit; SFRS2; PR264; SC-35; SC35; SFRS2A; SRp30b; Splicing component; 35 kDa; Splicing factor; arginine/serine-rich 2; SRSF2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Serine/Arginine Rich Splicing Factor 2 (SRSF2).; No significant cross-reactivity or interference between Serine/Arginine Rich Splicing Factor 2 (SRSF2) and analogues was observed.
• Sequence Length: 209

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serine/Arginine Rich Splicing Factor 2 (SRSF2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serine/Arginine Rich Splicing Factor 2 (SRSF2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SRSF2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine/Arginine Rich Splicing Factor 2 (SRSF2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serine/Arginine Rich Splicing Factor 2 (SRSF2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serine/Arginine Rich Splicing Factor 2 (SRSF2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serine/Arginine Rich Splicing Factor 2 (SRSF2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 306482646
• NCBI GeneID: 6427
• NCBI Accession #: NP_001182356.1
• NCBI GenBank Nucleotide #: NM_001195427.1
• UniProt Accession #: Q01130; B3KWD5; B4DN89; H0YG49
• Molecular Weight: 24,153 Da
• NCBI Official Full Name: serine/arginine-rich splicing factor 2
• NCBI Official Synonym Full Names: serine and arginine rich splicing factor 2
• NCBI Official Symbol: SRSF2
• NCBI Official Synonym Symbols: SC35; PR264; SC-35; SFRS2; SFRS2A; SRp30b
• NCBI Protein Information: serine/arginine-rich splicing factor 2
• UniProt Protein Name: Serine/arginine-rich splicing factor 2
• Protein Family: Serine/arginine-rich splicing factor
• UniProt Gene Name: SRSF2
• UniProt Synonym Gene Names: SFRS2; SC-35

NCBI Description

The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants encoding the same protein and one non-coding transcript variant have been found for this gene. In addition, a pseudogene of this gene has been found on chromosome 11. [provided by RefSeq, Sep 2010]

Uniprot Description

Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment.