Rabbit anti-Human H4 Polyclonal Antibody | anti-H4 antibody

H4 (K20 [Me2]) (HIST1H4A, H4/A, H4FA, Histone H4) (MaxLight 750)

Cat. Num. AAA6305293

• Gene Names: HIST2H4B; H4/o
• Reactivity: Human
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot, Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: H4; Polyclonal Antibody; H4 (K20 [Me2]) (HIST1H4A; H4/A; H4FA; Histone H4) (MaxLight 750); HIST1H4A; Histone H4; anti-H4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human dimethylated (K20) histone H4.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-H4 antibody: Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB), Dot Blot (DB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1-30 of human H4, UniProt Accession #P62805.
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis using MBS646539 on nitrocellulose membrane. 50ng of methylated peptide or non-methylated peptide per dot were adsorbed. Antibody working concentrations are 0.5ug/ml.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma reacted withMBS646539. Peroxidase-conjugated secondary antibody and DAB staining were used.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of MBS646539 using NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibody was used for the analysis.)

Western Blot (WB)

(Western Blot analysis of lysate (35ug per lane) from K562 cell line. MBS646539 was diluted 1:1000. Goat anti-rabbit IgG H&L-HRP at 1:5000 dilution was used as the secondary antibody.)

Related Product Information for anti-H4 antibody

Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones.

Product Categories/Family for anti-H4 antibody

Antibodies; Histones

NCBI and Uniprot Product Information

• NCBI GI #: 77539758
• NCBI GeneID: 554313
• NCBI Accession #: NP_001029249.1
• NCBI GenBank Nucleotide #: NM_001034077.4
• UniProt Accession #: P62805; P02304; P02305; Q6DRA9; Q6FGB8; Q6NWP7; A2VCL0
• Molecular Weight: 11,367 Da
• NCBI Official Full Name: histone H4
• NCBI Official Synonym Full Names: histone cluster 2, H4b
• NCBI Official Symbol: HIST2H4B
• NCBI Official Synonym Symbols: H4/o
• NCBI Protein Information: histone H4; histone 2, H4b
• UniProt Protein Name: Histone H4
• Protein Family: Histone
• UniProt Gene Name: HIST1H4A
• UniProt Synonym Gene Names: H4/A; H4FA
• UniProt Entry Name: H4_HUMAN

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Subunit structure: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

Subcellular location: Nucleus. Chromosome.

Post-translational modification: Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing. Ref.19 Ref.20 Ref.21 Ref.23 Ref.24 Ref.28Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4. Ref.28 Ref.36Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me). Ref.27 Ref.30Sumoylated, which is associated with transcriptional repression. Ref.22Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. Ref.35

Sequence similarities: Belongs to the histone H4 family.

Sequence caution: The sequence AAI28106.1 differs from that shown. Reason: Frameshift at position 3.