Western Blot (WB)
(Western blot analysis of lysates from HeLa cells, using ME1 Antibody. The lane on the right is blocked with the synthesized peptide.)
Rabbit ME1 Polyclonal Antibody | anti-ME1 antibody
ME1 polyclonal antibody
ELISA: 1/40,000
Western Blot (WB)
(Western blot analysis of lysates from HeLa cells, using ME1 Antibody. The lane on the right is blocked with the synthesized peptide.)
Western Blot (WB)
(Western blot analysis of lysates from HeLa cells, using ME1 Antibody. The lane on the right is blocked with the synthesized peptide.)
Western Blot (WB)
(Western blot analysis of the lysates from HeLa cells using ME1 antibody.)
Western Blot (WB)
(Western blot analysis of the lysates from HeLa cells using ME1 antibody.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq, Jul 2008]
Uniprot Description
ME1: a ubiquitous lipogenic enzyme of the malic enzymes family. Converts (S)-malate + NADP+ ?? pyruvate + CO2 + NADPH. Levels of ME1 and ATP-citrate lyase mRNA, another lipogenic enzyme, are coordinately increased 5-7 fold during the differentiation of 3T3-L1 preadipocytes into adipocytes. Up-regulated by short-term dietary restriction in mice. The cycling of pyruvate by ME1 generates cytosolic NADPH, whereas mitochondrial ME2 responds to elevated amino acids and serves to supply sufficient pyruvate for increased Krebs cycle flux when glucose is limiting.
Protein type: Carbohydrate Metabolism - pyruvate; EC 1.1.1.40; Oxidoreductase
Chromosomal Location of Human Ortholog: 6q12
Cellular Component: mitochondrion; cytosol
Molecular Function: malate dehydrogenase (decarboxylating) activity; malic enzyme activity; electron carrier activity; manganese ion binding; malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity; NADP binding; ADP binding; NAD binding; oxaloacetate decarboxylase activity
Biological Process: malate metabolic process; carbohydrate metabolic process; response to hormone stimulus; cellular lipid metabolic process; NADP biosynthetic process; protein tetramerization; response to carbohydrate stimulus
Research Articles on ME1
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Product Notes
The ME1 me1 (Catalog #AAA3001736) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ME1 polyclonal antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ME1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1/500-1/2000 ELISA: 1/40,000. Researchers should empirically determine the suitability of the ME1 me1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ME1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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