Rabbit anti-Human ME1 Polyclonal Antibody | anti-ME1 antibody

ME1 antibody

Cat. Num. AAA9408503

• Gene Names: ME1; MES; HUMNDME
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: Purified by antigen-affinity chromatography.
• Synonyms: ME1; Polyclonal Antibody; ME1 antibody; anti-ME1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified by antigen-affinity chromatography.
• Form/Format: Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Sequence Length: 572

• Applicable Applications for anti-ME1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500-1:3000; Immunofluorescence: 1:100-1:200
• Immunogen Type: Recombinant protein
• Immunogen Description: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 161 and 489 of ME1
• Target Name: ME1
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Testing Data

(Sample(30 ug whole cell lysate)A: A431B: HeLa S37.5% SDS PAGEPrimary antibody diluted at 1: 500)

Immunofluorescence (IF)

(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ME1 antibody at 1: 200 dilution.)

Related Product Information for anti-ME1 antibody

This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq]

Product Categories/Family for anti-ME1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4505143
• NCBI GeneID: 4199
• NCBI Accession #: NP_002386
• NCBI GenBank Nucleotide #: NM_002395.5
• Molecular Weight: 55,165 Da
• NCBI Official Full Name: NADP-dependent malic enzyme
• NCBI Official Synonym Full Names: malic enzyme 1, NADP(+)-dependent, cytosolic
• NCBI Official Symbol: ME1
• NCBI Official Synonym Symbols: MES; HUMNDME
• NCBI Protein Information: NADP-dependent malic enzyme
• UniProt Protein Name: NADP-dependent malic enzyme
• Protein Family: NADP-dependent malic enzyme
• UniProt Gene Name: ME1
• UniProt Synonym Gene Names: NADP-ME
• UniProt Entry Name: MAOX_HUMAN

NCBI Description

This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq, Jul 2008]

Uniprot Description

ME1: a ubiquitous lipogenic enzyme of the malic enzymes family. Converts (S)-malate + NADP+ ?? pyruvate + CO2 + NADPH. Levels of ME1 and ATP-citrate lyase mRNA, another lipogenic enzyme, are coordinately increased 5-7 fold during the differentiation of 3T3-L1 preadipocytes into adipocytes. Up-regulated by short-term dietary restriction in mice. The cycling of pyruvate by ME1 generates cytosolic NADPH, whereas mitochondrial ME2 responds to elevated amino acids and serves to supply sufficient pyruvate for increased Krebs cycle flux when glucose is limiting.

Protein type: Carbohydrate Metabolism - pyruvate; Oxidoreductase; EC 1.1.1.40

Chromosomal Location of Human Ortholog: 6q12

Cellular Component: mitochondrion; cytosol

Molecular Function: malate dehydrogenase (decarboxylating) activity; malic enzyme activity; electron carrier activity; manganese ion binding; malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity; NADP binding; ADP binding; NAD binding; oxaloacetate decarboxylase activity

Biological Process: malate metabolic process; carbohydrate metabolic process; response to hormone stimulus; NADP biosynthetic process; cellular lipid metabolic process; response to carbohydrate stimulus; protein tetramerization