Rabbit anti-Human Cyclin E Polyclonal Antibody | anti-CCNE1 antibody

Anti-Cyclin E Antibody, Rabbit Polyclonal

Cat. Num. AAA8104307

• Gene Names: CCNE1; CCNE
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: Cyclin E; Polyclonal Antibody; Anti-Cyclin E Antibody; Rabbit Polyclonal; CCNE1/Cyclin-E1 Antibody; Rabbit PAb; Antigen Affinity Purified; cyclin E1; Anti-CCNE Antibody; anti-CCNE1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human CCNE1/Cyclin-E1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CCNE1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000; IHC-P: 1:2000-1:10,000
• Immunogen: Recombinant Human CCNE1 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human CCNE1 (rh CCNE1). CCNE1 specific IgG was purified by human CCNE1 affinity chromatography .
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human CCNE1 in human placenta with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections. The image showing nucleus staining of decidual cells. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein.)

Western Blot (WB)

(Anti-CCNE1 rabbit polyclonal antibody at 1:500 dilutionLane A: U87-MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:47 kDaObserved band size:47 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-CCNE1 antibody

Cyclin E1 is a member of the highly conserved cyclin family and belongs to the E-type cyclin that functions as a regulator of S phase entry and progression in mammalian cells. Cyclin E1 serves as regulatory subunits that bind, activate, and provide substrate for its associated cyclin-dependent kinase2 (CDK2), whose activity is essential for cell cycle G1/S transition. Over expression of this encoding gene has been found in many tumors, which results in chromosome instability and by extension, induce tumorigenesis. This protein was also found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. In general, cyclin E1, as an activator of phospho-CDK2 (pCDK2), is important for cell cycle progression and is frequently overexpressed in cancer cells.

Product Categories/Family for anti-CCNE1 antibody

p53 Pathway

References

Honda R, et al. (2005) The structure of cyclin E1/CDK2: implications for CDK2 activation and CDK2-independent roles. The EMBO Journal. 24: 452-63.Geng Y, et al. (2007) Kinase-Independent Function of Cyclin E. 25(1): 127-39.

NCBI and Uniprot Product Information

• NCBI GI #: 17318559
• NCBI GeneID: 898
• NCBI Accession #: NP_001229.1
• NCBI GenBank Nucleotide #: NM_001238.2
• UniProt Accession #: P24864; Q14091; Q8NFG1; Q92501; Q9UD21; A8K684
• Molecular Weight: 47,077 Da
• NCBI Official Full Name: G1/S-specific cyclin-E1
• NCBI Official Synonym Full Names: cyclin E1
• NCBI Official Symbol: CCNE1
• NCBI Official Synonym Symbols: CCNE
• NCBI Protein Information: G1/S-specific cyclin-E1; cyclin Es; cyclin Et
• UniProt Protein Name: G1/S-specific cyclin-E1
• UniProt Gene Name: CCNE1
• UniProt Synonym Gene Names: CCNE
• UniProt Entry Name: CCNE1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available. [provided by RefSeq, Jul 2008]

Uniprot Description

CCNE1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Two alternatively spliced isoforms have been described.

Protein type: Nuclear receptor co-regulator; Activator; Cell cycle regulation

Chromosomal Location of Human Ortholog: 19q12

Cellular Component: nucleoplasm; cyclin-dependent protein kinase holoenzyme complex; nucleus; cytosol

Molecular Function: protein binding; androgen receptor binding; transcription coactivator activity; kinase activity; cyclin-dependent protein kinase regulator activity; protein kinase binding

Biological Process: G1/S-specific transcription in mitotic cell cycle; Wnt receptor signaling pathway; DNA replication initiation; cell division; regulation of cell cycle; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; mitotic cell cycle; regulation of protein kinase activity; protein amino acid phosphorylation; G1/S transition of mitotic cell cycle