Mouse anti-Human MDM4/MDMX Monoclonal Antibody | anti-MDM4/MDMX antibody

Anti-MDM4/MDMX Antibody (clone 3B9)

Cat. Num. AAA2401672

• Gene Names: MDM4; HDMX; MDMX; MRP1
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Protein A/G purified
• Synonyms: MDM4/MDMX; Monoclonal Antibody; Anti-MDM4/MDMX Antibody (clone 3B9); Mouse Monoclonal [clone 2D10F4] (IgG1) to Human MDM4/MDMX; MDM4; Mdm2-like p53-binding protein; MDMX; HDMX; MRP1; p53-binding protein Mdm4; Protein Mdm4; Double minute 4 protein; MDM4-related protein 1; Protein Mdmx; anti-MDM4/MDMX antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 3B9
• Specificity: Human MDM4/MDMX
• Purity/Purification: Protein A/G purified
• Form/Format: PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
• Sequence Length: 490

• Applicable Applications for anti-MDM4/MDMX antibody: Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IF: 1:100; IHC-P: 1:50; WB: 1:2000
• Immunogen: Full length human Recombinant protein of human MDM4(NP_002384) produced in HEK293T cell.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunofluorescence (IF)

(Anti-MDM4 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MDM4.)

Western Blot (WB)

(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MDM4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MDM4.)

Related Product Information for anti-MDM4/MDMX antibody

nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structuralsimilarity to p53-binding protein MDM2. Both proteins bind the p53 tumorsuppressor protein and inhibit its activity, and have been shown to beoverexpressed in a variety of human cancers. However, unlike MDM2 whichdegrades p53, this protein inhibits p53 by binding its transcriptionalactivation domain.

NCBI and Uniprot Product Information

• NCBI GI #: 88702791
• NCBI GeneID: 4194
• NCBI Accession #: NP_002384.2
• NCBI GenBank Nucleotide #: NM_002393.4
• UniProt Accession #: O15151; Q2M2Y2; Q32SL2; Q6GS18; Q8IV83
• Molecular Weight: 49,541 Da
• NCBI Official Full Name: protein Mdm4 isoform 1
• NCBI Official Synonym Full Names: MDM4, p53 regulator
• NCBI Official Symbol: MDM4
• NCBI Official Synonym Symbols: HDMX; MDMX; MRP1
• NCBI Protein Information: protein Mdm4
• UniProt Protein Name: Protein Mdm4
• UniProt Gene Name: MDM4
• UniProt Synonym Gene Names: MDMX

NCBI Description

This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions.