Rabbit anti-Human MDC1 Polyclonal Antibody | anti-MDC1 antibody

MDC1 Conjugated Antibody

Cat. Num. AAA9447624

• Gene Names: NFAT5; NFATZ; OREBP; NF-AT5; NFATL1; TONEBP
• Reactivity: Human
• Applications: Most Applications
• Synonyms: MDC1; Polyclonal Antibody; MDC1 Conjugated Antibody; NFBD1; anti-MDC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total MDC1 protein.
• Form/Format: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide

• Applicable Applications for anti-MDC1 antibody: Most Applications
• Application Notes: Suggested Dilution:; AF350 conjugated: most applications: 1:50-1:250; AF405 conjugated: most applications: 1:50-1:250; AF488 conjugated: most applications: 1:50-1:250; AF555 conjugated: most applications: 1:50-1:250; AF594 conjugated: most applications: 1:50-1:250; AF647 conjugated: most applications: 1:50-1:250; AF680 conjugated: most applications: 1:50-1:250; AF750 conjugated: most applications: 1:50-1:250; Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1:50-1:1000
• Immunogen: Synthetic peptide corresponding to a region derived from internal residues of human Mediator of DNA-damage checkpoint 1
• Conjugation: Biotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
• Excitation Emission: AF350: 346nm/442nm
• Preparation and Storage: Store at 4 degree C for 6 months.

Related Product Information for anti-MDC1 antibody

The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.

NCBI and Uniprot Product Information

• NCBI GI #: 5729945
• NCBI GeneID: 10725
• NCBI Accession #: NP_006590
• NCBI GenBank Nucleotide #: NM_006599
• UniProt Accession #: Q14676; O94916
• Molecular Weight: 166kDa
• NCBI Official Full Name: nuclear factor of activated T-cells 5 isoform c
• NCBI Official Synonym Full Names: nuclear factor of activated T cells 5
• NCBI Official Symbol: NFAT5
• NCBI Official Synonym Symbols: NFATZ; OREBP; NF-AT5; NFATL1; TONEBP
• NCBI Protein Information: nuclear factor of activated T-cells 5
• UniProt Protein Name: Mediator of DNA damage checkpoint protein 1
• Protein Family: Mediator of DNA damage checkpoint protein
• UniProt Gene Name: MDC1
• UniProt Synonym Gene Names: KIAA0170; NFBD1
• UniProt Entry Name: MDC1_HUMAN

NCBI Description

The product of this gene is a member of the nuclear factors of activated T cells family of transcription factors. Proteins belonging to this family play a central role in inducible gene transcription during the immune response. This protein regulates gene expression induced by osmotic stress in mammalian cells. Unlike monomeric members of this protein family, this protein exists as a homodimer and forms stable dimers with DNA elements. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MDC1: Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: nucleoplasm; focal adhesion; chromosome; nucleus

Molecular Function: protein C-terminus binding; protein binding

Biological Process: intra-S DNA damage checkpoint; double-strand break repair; DNA repair; double-strand break repair via homologous recombination