Western Blot (WB)
(Fig-1: Western blot analysis of IKKe antibody (Clone: ABM13C7) was tested at 2 ug/ml on Jurkat, NIH 3T3 and HepG2 lysates using Goat anti Mouse IgG HRP conjugated secondary antibody (11-301) with 1: 5000 dilution.)
Goat anti Mouse IgG (H+L) Secondary Antibody | IgG secondary antibody
Peroxidase conjugated Goat anti Mouse IgG (H+L)
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
Afinity Chromatography
Synonyms
Goat anti Mouse IgG (H+L); Polyclonal Secondary Antibody; Peroxidase conjugated Goat anti Mouse IgG (H+L); Antibody to Mouse IgG (H+L); Anti-Mouse IgG (H+L) Antibody; Mouse IgG (H+L) Antibody; IgG secondary antibody
Host
Goat
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Afinity Chromatography
Applicable Applications for IgG secondary antibody
ELISA (EIA), Western Blot (WB)
Application Notes
WB: 1: 5,000-1: 20,000
Antigen
Mouse IgG (H+L)
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
Western Blot (WB)
(Fig-1: Western blot analysis of IKKe antibody (Clone: ABM13C7) was tested at 2 ug/ml on Jurkat, NIH 3T3 and HepG2 lysates using Goat anti Mouse IgG HRP conjugated secondary antibody (11-301) with 1: 5000 dilution.)
Western Blot (WB)
(Fig-1: Western blot analysis of IKKe antibody (Clone: ABM13C7) was tested at 2 ug/ml on Jurkat, NIH 3T3 and HepG2 lysates using Goat anti Mouse IgG HRP conjugated secondary antibody (11-301) with 1: 5000 dilution.)
Related Product Information for IgG secondary antibody
Secondary antibodies are antibodies that bind to primary antibodies or antibody fragments. They are typically labeled with probes that make them useful for detection, purification or cell sorting applications. Secondary antibodies may be polyclonal or monoclonal, and are available with specificity for whole Ig molecules or antibody fragments such as the Fc or Fab regions. Secondary antibodies are selected according to the source of the primary antibody, the class of the primary antibody (e.g., IgG or IgM), and the kind of label which is preferred. Identifying the optimal secondary antibody is normally done through trial and error method. Secondary antibodies are used in all types of immunoassays, most often in Western blot, immunohistochemistry, and immunocytochemistry, and occasionally in immunoprecipitation. Basic research, clinical analysis, and disease identification also use secondary antibodies in ELISA and flow cytometry assays. They are also useful for cell sorting, fluorescence activated cell sorting, FACS.
Product Categories/Family for IgG secondary antibody
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Product Notes
The IgG (Catalog #AAA668922) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Peroxidase conjugated Goat anti Mouse IgG (H+L) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Goat anti Mouse IgG (H+L) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB: 1: 5,000-1: 20,000. Researchers should empirically determine the suitability of the IgG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Goat anti Mouse IgG (H+L), Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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