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Western Blot (WB) (Western blot analysis of CHSY3 using NIH-3T3 whole cell lysates)

Rabbit CHSY3 Polyclonal Antibody | anti-CHSY3 antibody

CHSY3 Antibody

Gene Names
CHSY3; CSS3; CHSY2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CHSY3; Polyclonal Antibody; CHSY3 Antibody; Carbohydrate synthase 2; Chondroitin glucuronyltransferase 3; Chondroitin sulfate synthase 3; Chondroitin synthase 2; CHSS3_HUMAN; ChSy-2; CHSY2; Chsy3; CSS3; Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase II; N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase 3; N-acetylgalactosaminyltransferase 3; anti-CHSY3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
CHSY3 antibody detects endogenous levels of total CHSY3
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
882
Applicable Applications for anti-CHSY3 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human CHSY3
Subcellular Location
Golgi Apparatus > Golgi Stack Membrane.
Tissue Specificity
Detected at low levels in brain, cerebral cortex, uterus and small intestine.
Predicted Cross Reactivity
Bovine, Dog
Similarity
Bovine (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of CHSY3 using NIH-3T3 whole cell lysates)

Western Blot (WB) (Western blot analysis of CHSY3 using NIH-3T3 whole cell lysates)

Western Blot (WB)

(Western blot analysis of extracts from rat brain, mouse brain, using CHSY3 Antibody.)

Western Blot (WB) (Western blot analysis of extracts from rat brain, mouse brain, using CHSY3 Antibody.)
Related Product Information for anti-CHSY3 antibody
Function: Has both beta-1, 3-glucuronic acid and beta-1, 4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. Specific activity is much reduced compared to CHSY1.
Similarity: Belongs to the chondroitin N-acetylgalactosaminyltransferase family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 100 kDa
Predicted: 101 kDa
NCBI Official Full Name
chondroitin sulfate synthase 3
NCBI Official Synonym Full Names
chondroitin sulfate synthase 3
NCBI Official Symbol
CHSY3
NCBI Official Synonym Symbols
CSS3; CHSY2
NCBI Protein Information
chondroitin sulfate synthase 3
UniProt Protein Name
Chondroitin sulfate synthase 3
UniProt Gene Name
CHSY3
UniProt Synonym Gene Names
CHSY2; CSS3; ChSy-2

NCBI Description

CSS3 is a glycosyltransferase that has both glucuronyltransferase and N-acetylgalactosaminyltransferase activities (Yada et al., 2003 [PubMed 12907687]).[supplied by OMIM, Mar 2008]

Uniprot Description

Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. Specific activity is much reduced compared to CHSY1.

Research Articles on CHSY3

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Product Notes

The CHSY3 chsy3 (Catalog #AAA9609695) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CHSY3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CHSY3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the CHSY3 chsy3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHSY3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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