Rabbit anti-Human MDC1 Polyclonal Antibody | anti-MDC1 antibody

MDC1 Polyclonal Antibody

Cat. Num. AAA9128514

• Gene Names: MDC1; NFBD1
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purification
• Synonyms: MDC1; Polyclonal Antibody; MDC1 Polyclonal Antibody; anti-MDC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 1,066

• Applicable Applications for anti-MDC1 antibody: Western Blot (WB)
• Application Notes: WB 1:500 - 1:2000
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human MDC1
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using MDC1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 5s.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung cancer using MDC1 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon carcinoma using MDC1 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human prostate using MDC1 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human prostate cancer using MDC1 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human esophagus using MDC1 antibody at dilution of 1:100 (40x lens).)

Related Product Information for anti-MDC1 antibody

The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.

NCBI and Uniprot Product Information

• NCBI GI #: 132626688
• NCBI GeneID: 9656
• NCBI Accession #: NP_055456.2
• NCBI GenBank Nucleotide #: NM_014641.2
• UniProt Accession #: Q14676; Q0EFC2; Q2L6H7; Q2TAZ4; Q5JP55; Q5JP56; Q5ST83; A2AB04; A2BF04; A2RRA8; A7YY86; B0S8A2
• Molecular Weight: 227kDa
• NCBI Official Full Name: mediator of DNA damage checkpoint protein 1
• NCBI Official Synonym Full Names: mediator of DNA-damage checkpoint 1
• NCBI Official Symbol: MDC1
• NCBI Official Synonym Symbols: NFBD1
• NCBI Protein Information: mediator of DNA damage checkpoint protein 1
• UniProt Protein Name: Mediator of DNA damage checkpoint protein 1
• Protein Family: Mediator of DNA damage checkpoint protein
• UniProt Gene Name: MDC1
• UniProt Synonym Gene Names: KIAA0170; NFBD1
• UniProt Entry Name: MDC1_HUMAN

NCBI Description

The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. [provided by RefSeq, Jul 2008]

Uniprot Description

MDC1: Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: nucleoplasm; focal adhesion; chromosome; nucleus

Molecular Function: protein C-terminus binding; protein binding

Biological Process: intra-S DNA damage checkpoint; double-strand break repair; DNA repair; double-strand break repair via homologous recombination