Rabbit anti-Human, Mouse MCT-1 Polyclonal Antibody | anti-MCT-1 antibody

MCT-1, NT (Malignant T cell Amplified Sequence 1, MCT1, MCTS1, FLJ39637, Multiple Copies T-cell Malignancies, Oncogene MCT-1) (APC)

Cat. Num. AAA6487415

• Gene Names: MCTS1; MCT1; MCT-1
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: MCT-1; Polyclonal Antibody; NT (Malignant T cell Amplified Sequence 1; MCT1; MCTS1; FLJ39637; Multiple Copies T-cell Malignancies; Oncogene MCT-1) (APC); anti-MCT-1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MCTS1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-MCT-1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human MCTS1 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MCT-1 antibody (N-term) in mouse bladder tissue lysates (35ug/lane). MCTS1 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-MCT-1 antibody

Antibodies; Oncoproteins

References

1. Kasiappan, R., Mol. Cancer Res. 7(4), 536-548 (2009). 2. Mazan-Mamczarz, K., Leuk. Res. 33(3), 474-482 (2009). 3. Shi, B., J. Cell. Biochem. 90(1), 68-79 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 7662502
• NCBI GeneID: 28985
• NCBI Accession #: NP_054779
• NCBI GenBank Nucleotide #: NM_014060.2
• UniProt Accession #: Q9ULC4; Q502X6; B4DGY2
• Molecular Weight: 22.9 kDa (204aa) confirmed by MALDI-TOF
• NCBI Official Full Name: malignant T-cell-amplified sequence 1 isoform 1
• NCBI Official Synonym Full Names: MCTS1, re-initiation and release factor
• NCBI Official Symbol: MCTS1
• NCBI Official Synonym Symbols: MCT1; MCT-1
• NCBI Protein Information: malignant T-cell-amplified sequence 1
• UniProt Protein Name: Malignant T-cell-amplified sequence 1
• UniProt Gene Name: MCTS1
• UniProt Synonym Gene Names: MCT1; MCT-1
• UniProt Entry Name: MCTS1_HUMAN

Uniprot Description

MCTS1: Anti-oncogene that play a role in cell cycle regulation; decreases cell doubling time and anchorage-dependent growth; shortens the duration of G1 transit time and G1/S transition. When constituvely expressed, increases CDK4 and CDK6 kinases activity and CCND1/cyclin D1 protein level, as well as G1 cyclin/CDK complex formation. Plays a role as translation enhancer; Recruits the density-regulated protein/DENR and binds to the cap complex of the 5'-terminus of mRNAs, subsequently altering the mRNA translation profile; Up-regulates protein levels of BCL2L2, TFDP1, MRE11A, CCND1 and E2F1, while mRNA levels remains constant. Hyperactivates DNA damage signaling pathway; increased gamma- irradiation-induced phosphorylation of histone H2AX, and induces damage foci formation. Increases the overall number of chromosomal abnormalities such as larger chromosomes formation and multiples chromosomal fusions when overexpressed in gamma-irradiated cells. May play a role in promoting lymphoid tumor development: lymphoid cell lines overexpressing MCTS1 exhibit increased growth rates and display increased protection against apoptosis. May contribute to the pathogenesis and progression of breast cancer via promotion of angiogenesis through the decline of inhibitory THBS1/thrombospondin-1, and inhibition of apoptosis. Involved in the process of proteasome degradation to down-regulate Tumor suppressor p53/TP53 in breast cancer cell; Positively regulates phosphorylation of MAPK1 and MAPK3. Belongs to the MCTS1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor

Chromosomal Location of Human Ortholog: Xq24

Cellular Component: cytoplasm; plasma membrane

Molecular Function: translation initiation factor activity

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; regulation of growth; positive regulation of cell proliferation; ribosome disassembly; cell cycle; response to DNA damage stimulus; formation of translation preinitiation complex