Rabbit MCP3 Polyclonal Antibody | anti-CCL7 antibody

Anti-MCP3 Antibody, Rabbit Polyclonal

Cat. Num. AAA8102761

• Gene Names: Ccl7; fic; marc; mcp3; MCP-3; Scya7
• Reactivity: Mouse; Predicted to React with: Rat (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: MCP3; Polyclonal Antibody; Anti-MCP3 Antibody; Rabbit Polyclonal; MCP3/CCL7 Antibody; Rabbit PAb; Antigen Affinity Purified; chemokine (C-C motif) ligand 7; Anti-fic Antibody; Anti-marc Antibody; Anti-MCP-3 Antibody; Anti-mcp3 Antibody; Anti-Scya7 Antibody; anti-CCL7 antibody

• Host: Rabbit
• Reactivity: Mouse; Predicted to React with: Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse MCP3
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-CCL7 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to the center region of the Mouse MCP3/CCL7
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse MCP3/CCL7, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-Ccl7 rabbit polyclonal antibody at 1:500 dilution Lane A: K562 Whole Cell Lysate Lane B: Mouse small intestine tissue lysate Lane C: Mouse pancreas tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:11 kDa)

Related Product Information for anti-CCL7 antibody

Chemokines are a family of small chemotactic cytokines, or proteins secreted by cells. Chemokines share the same structure similarities such as small size, and the presence of four cysteine residues in conserved locations in order to form their 3-dimensional shape. Some of the chemokines are considered pro-inflammatory which can be induced to recruit cells of the immune system to a site of infection during an immune response, while others are considered homeostatic and are implied in controlling the migration of cells during normal processes of tissue maintenance and development. There are four members of the chemokine family: C-C kemokines, C kemokines, CXC kemokines and CX3C kemokines. The C-C kemokines have two cysteines nearby the amino terminus. There have been at least 27 distinct members of this subgroup reported for mammals, called C-C chemokine ligands-1 to 28. Chemokine ligand 7(CCL7), also known as MCP-3, is a isform of the C-C chemokine subfamily of the chemokine family which is produced by certain tumor cells and by macrophages. It also own two adjacent N-terminal cysteine residues. Chemokine ligand 7(CCL7) spacifically attracts monocytes, and regulates macrophage function.

Product Categories/Family for anti-CCL7 antibody

IL17 signaling pathway

References

Laing KJ, et al. (2004) Chemokines. Developmental and comparative immunology. 28(5): 443-60.Cocchi F, et al. (1995) Identification of RANTES, MIP-1a, and MIP-1b as the major HIV-suppressive factor produced by CD8+ T cells. Science. 270 (5243): 1811-5.Michalec L, et al. (2002) CCL7 and CXCL10 Orchestrate Oxidative Stress-Induced Neutrophilic Lung Inflammation. The Journal of Immunology. 168: 846-52.Wetzel K, et al. (2007) MCP-3 (CCL7) delivered by parvovirus MVMp reduces tumorigenicity of mouse melanoma cells through activation of T lymphocytes and NK cells.International Journal of Cancer. 120(6):1364-71.

NCBI and Uniprot Product Information

• NCBI GI #: 7305463
• NCBI GeneID: 20306
• NCBI Accession #: NP_038682.1
• NCBI GenBank Nucleotide #: NM_013654.3
• UniProt Accession #: Q03366
• Molecular Weight: 10,999 Da
• NCBI Official Full Name: C-C motif chemokine 7
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 7
• NCBI Official Symbol: Ccl7
• NCBI Official Synonym Symbols: fic; marc; mcp3; MCP-3; Scya7
• NCBI Protein Information: C-C motif chemokine 7; RANTES/sis homolog; intercrine/chemokine MARC; monocyte chemoattractant protein 3; monocyte chemotactic protein 3; small inducible cytokine A7; small-inducible cytokine A7
• UniProt Protein Name: C-C motif chemokine 7
• Protein Family: C-C motif chemokine
• UniProt Gene Name: Ccl7
• UniProt Synonym Gene Names: Fic; Mcp3; Scya7; MCP-3
• UniProt Entry Name: CCL7_MOUSE

Uniprot Description

CCL7: Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; CCR2 chemokine receptor binding; chemokine activity; cytokine activity

Biological Process: immune response; chemotaxis; inflammatory response