Rabbit anti-Human MAP2K4 Polyclonal Antibody | anti-MAP2K4 antibody

MAP2K4, phosphorylated (Ser80) (Dual Specificity Mitogen-activated Protein Kinase Kinase 4, MAP Kinase Kinase 4, MAPKK 4, MAPKK4, MKK4, JNK-activating Kinase 1, c-Jun N-terminal Kinase Kinase 1, JNKK1, JNKK, MAPK/ERK Kinase 4, MEK 4, MEK4, PRKMK4, SAPK/ER

Cat. Num. AAA646915

• Gene Names: MAP2K4; JNKK; MEK4; MKK4; SEK1; SKK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: MAP2K4; Polyclonal Antibody; phosphorylated (Ser80) (Dual Specificity Mitogen-activated Protein Kinase Kinase 4; MAP Kinase Kinase 4; MAPKK 4; MAPKK4; MKK4; JNK-activating Kinase 1; c-Jun N-terminal Kinase Kinase 1; JNKK1; JNKK; MAPK/ERK Kinase 4; MEK 4; MEK4; PRKMK4; SAPK/ER; Anti -MAP2K4; anti-MAP2K4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MAP2K4 when phosphorylated at Ser80. Species sequence homology: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-MAP2K4 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
• Immunogen: Synthetic phosphopeptide corresponding to 80aa from human MAP2K4 around the phosphorylation site of serine 80 (T-H-SP-I-E) (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Kidney (formalin-fixed, paraffin-embedded) stained with MAP2K4 antibody at 10ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-MAP2K4 antibody

MAP2K4 is a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. MAP2K4 is phosphorylated, and thus activated by MAP3K1/MEKK. Knockout studies in mice suggest roles for this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver.

Product Categories/Family for anti-MAP2K4 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 49168612
• NCBI GeneID: 6416
• UniProt Accession #: P45985; Q5U0B8; Q6FHX4; Q6P9H2; Q6PIE6; B2R7N7; B3KYB2; D3DTS5
• Molecular Weight: 44,288 Da
• NCBI Official Full Name: MAP2K4
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 4
• NCBI Official Symbol: MAP2K4
• NCBI Official Synonym Symbols: JNKK; MEK4; MKK4; SEK1; SKK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 4; MEK 4; MAPKK 4; MAPK/ERK kinase 4; SAPK/ERK kinase 1; MAP kinase kinase 4; JNK-activated kinase 1; JNK activating kinase 1; JNK-activating kinase 1; c-Jun N-terminal kinase kinase 1; stress-activated protein kinase kinase 1
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 4
• Protein Family: Dual specificity mitogen-activated protein kinase kinase
• UniProt Gene Name: MAP2K4
• UniProt Synonym Gene Names: JNKK1; MEK4; MKK4; PRKMK4; SEK1; SERK1; SKK1; MAP kinase kinase 4; MAPKK 4; MEK 4; SEK1; SAPK kinase 1; SAPKK-1; SAPKK1; JNKK
• UniProt Entry Name: MP2K4_HUMAN

NCBI Description

This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

Function: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14. Ref.2

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation: Activated in response to a variety of cellular stresses, including UV and gamma-irradiation, heat shock, hyperosmolarity, T-cell receptor stimulation, peroxide and inflammatory cytokines. Also activated by developmental cues. MAP2K4/MKK4 is activated by the majority of MKKKs, such as MAP3K5/ASK1, MAP3K1/MEKK1, MAP3K7/TAK1, MAP3K10/MLK2, MAP3K11/MLK3, MAP3K12/DLK and MAP3K13/LZK. Ref.11

Subunit structure: Interacts with SPAG9

By similarity. Interacts (via its D domain) with its substrates MAPK8/JNK1, MAPK9/JNK2, MAPK10/JNK3, MAPK11 and MAPK14. Interacts (via its DVD domain) with MAP3Ks activators like MAP3K1/MEKK1 and MAP3K11/MLK3. Interacts with ARRB1, ARRB2 and MAPK8IP3/JIP3. Ref.11 Ref.13 Ref.14 Ref.15 Ref.19

Subcellular location: Cytoplasm

By similarity. Nucleus

By similarity.

Tissue specificity: Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.

Domain: The DVD domain (residues 364-387) contains a conserved docking site and is found in the mammalian MAP kinase kinases (MAP2Ks). The DVD sites bind to their specific upstream MAP kinase kinase kinases (MAP3Ks) and are essential for activation. Ref.15 Ref.16The D domain (residues 34-52) contains a conserved docking site and is required for the binding to MAPK substrates. Ref.15 Ref.16

Post-translational modification: Activated by phosphorylation on Ser-257 and Thr-261 by MAP kinase kinase kinases (MAP3Ks).

Sequence similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.Contains 1 protein kinase domain.