anti-Human MBD4 Polyclonal Antibody | anti-MBD4 antibody

MBD4 (Methyl-CpG-binding Domain Protein 4, Methyl-CpG-binding Endonuclease 1, Methyl-CpG-binding Protein MBD4, Mismatch-specific DNA N-glycosylase, MED1)

Cat. Num. AAA612334

• Gene Names: MBD4; MED1
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: MBD4; Polyclonal Antibody; MBD4 (Methyl-CpG-binding Domain Protein 4; Methyl-CpG-binding Endonuclease 1; Methyl-CpG-binding Protein MBD4; Mismatch-specific DNA N-glycosylase; MED1); Anti -MBD4 (Methyl-CpG-binding Domain Protein 4; anti-MBD4 antibody

• Host: Host: Rabbit; Source: Human
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MBD4 (Methyl CpG Binding Protein 4)
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-MBD4 antibody: Western Blot (WB)
• Application Notes: Can be used for detection of MBD4 by Western blot. A protein of 64kD can be detected in HL60 cells. It has not been tested in other applications. Western blot analysis of MBD4 in HL-60 cell lysate with anti-MBD4 pcAb. A protein band of approximate molecular weight of 64kD was detected.; Dilution: Western blot: 2ug/ml.
• Immunogen: Synthetic peptides corresponding to amino acids 268-282 and 337-352 of human MBD4.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of MBD4 in HL-60 cell lysate with anti-MBD4 pcAb,MBS612334. A protein band of ~MW of 64kD was detected.)

Related Product Information for anti-MBD4 antibody

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (1). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (2). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (3). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (4).

Product Categories/Family for anti-MBD4 antibody

Antibodies; Abs to DNA Replication; Repair

NCBI and Uniprot Product Information

• NCBI GI #: 4505121
• NCBI GeneID: 8930
• NCBI Accession #: NP_003916.1
• NCBI GenBank Nucleotide #: NM_003925.1
• UniProt Accession #: O95243; Q7Z4T3; Q96F09; D3DNC3; D3DNC4; Q2MD36
• Molecular Weight: 66,051 Da
• NCBI Official Full Name: methyl-CpG-binding domain protein 4
• NCBI Official Synonym Full Names: methyl-CpG binding domain protein 4
• NCBI Official Symbol: MBD4
• NCBI Official Synonym Symbols: MED1
• NCBI Protein Information: methyl-CpG-binding domain protein 4; OTTHUMP00000215413; OTTHUMP00000215522; OTTHUMP00000215523; OTTHUMP00000215694; G/T mismatch glycosylase; G/U mismatch glycosylase; methyl-CpG-binding protein MBD4; 3,N(4)-ethenocytosine glycosylase; methyl-CpG-binding endonuclease 1; mismatch-specific DNA N-glycosylase; putative methyl-CpG binding protein; G/5-fluorouracil mismatch glycosylase with biphasic kinetics
• UniProt Protein Name: Methyl-CpG-binding domain protein 4
• Protein Family: Methyl-CpG-binding domain protein
• UniProt Gene Name: MBD4
• UniProt Synonym Gene Names: MED1
• UniProt Entry Name: MBD4_HUMAN