Rabbit MBD4 Polyclonal Antibody | anti-MBD4 antibody

MBD4 Antibody

Cat. Num. AAA840448

• Gene Names: MBD4; MED1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: MBD4; Polyclonal Antibody; MBD4 Antibody; Methyl-CpG-binding domain protein 4; Methyl-CpG-binding endonuclease 1; Methyl-CpG-binding protein; Mismatch-specific DNA N-glycosylase; MED1; anti-MBD4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) affinity purified rabbit anti-MBD4 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 574

• Applicable Applications for anti-MBD4 antibody: Western Blot (WB)
• Application Notes: The antibody can be used in Western Blot analysis (0.5-4 ug/ml). However, the optimal concentrations should be determined individually.
• Immunogen: Synthetic peptide surrounding amino acid 474 of mouse MBD4
• Positive Control: Jurkat cell lysates
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-MBD4 antibody

Background: MBD4 (methyl-CpG-binding domain protein 4) belongs to the MBD family of nuclear proteins that bind to methylated CpG sites and represses gene transcription. Human proteins MECP2, MBD1, MBD2, and MBD4, except MBD3 bind specifically to methylated DNA. MBD4 exhibits thymine glycosylase activity and is known to play an important function in DNA mismatch repair and apoptosis.

Product Categories/Family for anti-MBD4 antibody

Epigenetics; Antibodies and PeptidesAntibodies & Supporting Tools; Primary Antibodies (A-Z) Molecular Biology Tools; Molecular Biology Antibodies & Blocking Peptides

NCBI and Uniprot Product Information

• NCBI GI #: 442796450
• NCBI GeneID: 8930
• NCBI Accession #: NP_001263199.1
• NCBI GenBank Nucleotide #: NM_001276270.1
• UniProt Accession #: O95243; Q2MD36; Q7Z4T3; Q96F09; B4DZN2; D3DNC3; D3DNC4; E9PEE4
• Molecular Weight: 30,316 Da
• NCBI Official Full Name: methyl-CpG-binding domain protein 4 isoform 2
• NCBI Official Synonym Full Names: methyl-CpG binding domain 4 DNA glycosylase
• NCBI Official Symbol: MBD4
• NCBI Official Synonym Symbols: MED1
• NCBI Protein Information: methyl-CpG-binding domain protein 4
• UniProt Protein Name: Methyl-CpG-binding domain protein 4
• Protein Family: Methyl-CpG-binding domain protein
• UniProt Gene Name: MBD4
• UniProt Synonym Gene Names: MED1
• UniProt Entry Name: MBD4_HUMAN

NCBI Description

The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains an MBD domain at the N-terminus that functions both in binding to methylated DNA and in protein interactions and a C-terminal mismatch-specific glycosylase domain that is involved in DNA repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]

Uniprot Description

MBD4: Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; DNA repair, damage; EC 3.2.2.-; Deoxyribonuclease

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: nucleoplasm; cytoplasm; chromatin; nucleus

Molecular Function: protein binding; satellite DNA binding; pyrimidine-specific mismatch base pair DNA N-glycosylase activity; endodeoxyribonuclease activity

Biological Process: response to radiation; DNA damage response, signal transduction resulting in induction of apoptosis; base-excision repair, AP site formation; depyrimidination; base-excision repair; mitotic cell cycle G2/M transition DNA damage checkpoint; DNA repair; DNA catabolic process, endonucleolytic