Rabbit MBD2a Polyclonal Antibody | anti-Mbd2 antibody

MBD2a (Methyl CpG Binding Domain Protein 2a, Demethylase, DMTase, DKFZp586O0821, MBD2, Methyl CpG Binding Domain Protein 2 Isoform 1, Methyl CpG Binding Protein MBD2, NY-CO-41)

Cat. Num. AAA6003364

• Gene Names: Mbd2; MBD2a
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: MBD2a; Polyclonal Antibody; MBD2a (Methyl CpG Binding Domain Protein 2a; Demethylase; DMTase; DKFZp586O0821; MBD2; Methyl CpG Binding Domain Protein 2 Isoform 1; Methyl CpG Binding Protein MBD2; NY-CO-41); Anti -MBD2a (Methyl CpG Binding Domain Protein 2a; anti-Mbd2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat MBD2a. Species Crossreactivity: human and mouse.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 5mM EDTA, 0.01% thimerosal, 30% glycerol.

• Applicable Applications for anti-Mbd2 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 0.5-4ug/ml
• Immunogen: Synthetic peptide corresponding to aa164 from rat MBD2a.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MBD2a using Jurkat cell lysate.)

Related Product Information for anti-Mbd2 antibody

The MBD family of proteins includes MeCP2, MBD1, MBD2, MBD3 and MBD4. MBD2 is highly similar to MBD3 but only MBD2 targets the MeCP1 complex to methylate DNA. MBD2 has two isoforms, MBD2a and MBD2b. MBD2a is a 414aa protein, whereas MBD2b lacks the 152aa N-terminal extension. MBD2a interacts with RNA helicase A to enhance CREB-dependent gene exptression.

Product Categories/Family for anti-Mbd2 antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 218931229
• NCBI GeneID: 17191
• NCBI Accession #: NP_034903.2
• NCBI GenBank Nucleotide #: NM_010773.2
• UniProt Accession #: Q9Z2E1; Q811D9; Q9Z2D9; E9QMV9
• Molecular Weight: 43,501 Da
• NCBI Official Full Name: methyl-CpG-binding domain protein 2
• NCBI Official Synonym Full Names: methyl-CpG binding domain protein 2
• NCBI Official Symbol: Mbd2
• NCBI Official Synonym Symbols: MBD2a
• NCBI Protein Information: methyl-CpG-binding domain protein 2; methyl-CpG-binding protein MBD2
• UniProt Protein Name: Methyl-CpG-binding domain protein 2
• Protein Family: Methyl-CpG-binding domain protein
• UniProt Gene Name: Mbd2
• UniProt Entry Name: MBD2_MOUSE

Uniprot Description

MBD2: Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. May enhance the activation of some unmethylated cAMP-responsive promoters. 2 isoforms of the human protein are produced by alternative splicing.

Cellular Component: heterochromatin; protein complex; cytoplasm; nuclear chromatin; histone deacetylase complex; nucleolus; chromatin; nucleus

Molecular Function: mRNA binding; protein domain specific binding; protein binding; siRNA binding; nucleosomal DNA binding; DNA binding; methyl-CpG binding; chromatin binding

Biological Process: cellular protein complex assembly; transcription, DNA-dependent; regulation of transcription, DNA-dependent; maternal behavior; ATP-dependent chromatin remodeling; negative regulation of transcription from RNA polymerase II promoter; positive regulation of Wnt receptor signaling pathway; regulation of cell proliferation