anti-Human MBD2 Polyclonal Antibody | anti-MBD2 antibody

Anti-MBD2 Antibody

Cat. Num. AAA177943

• Gene Names: MBD2; DMTase; NY-CO-41
• Reactivity: Human
• Applications: Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: MBD2; Polyclonal Antibody; Anti-MBD2 Antibody; Methyl-CpG-binding domain protein 2; DEMETHYLASE; DKFZp586O0821; DMTase; MBD 2; Mbd2; MBD2_HUMAN; MBD2a; Methyl CpG binding domain protein 2; Methyl CpG binding protein MBD2; Methyl-CpG-binding protein MBD2; NY CO 41; methyl-CpG binding domain protein 2; anti-MBD2 antibody

• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 411

• Applicable Applications for anti-MBD2 antibody: Western Blot (WB)
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml
• Immunogen: E Coli-derived human MBD2 recombinant protein (Position: W159-A411). Human MBD2 shares 98% amino acid (aa) sequence identity with mouse MBD2.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- MBD2 antibody, Western blottingAll lanes: Anti MBD2 at 0.5ug/mlWB: Recombinant Human MBD2 Protein 0.5ngPredicted bind size: 40KDObserved bind size: 40KD)

Western Blot (WB)

(Anti- MBD2 antibody, Western blottingAll lanes: Anti MBD2 at 0.5ug/mlLane 1: SGC Whole Cell Lysate at 40ugLane 2: HELA Whole Cell Lysate at 40ugLane 3: JURKAT Whole Cell Lysate at 40ugLane 4: K562 Whole Cell Lysate at 40ugPredicted bind size: 47KDObserved bind size: 47KD)

Related Product Information for anti-MBD2 antibody

Description: Rabbit IgG polyclonal antibody for Methyl-CpG-binding domain protein 2(MBD2) detection. Tested with WB in Human.

Background: Methyl-CpG-binding domain protein 2 is a protein that in humans is encoded by the MBD2 gene. It is mapped to 18q21.2. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG-binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.

References

1. Hendrich B, Abbott C, McQueen H, Chambers D, Cross S, Bird A (September 1999). "Genomic structure and chromosomal mapping of the murine and human Mbd1, Mbd2, Mbd3, and Mbd4 genes". Mamm Genome 10 (9): 906-12. 2. Hendrich, B., Bird, A. Identification and characterization of a family of mammalian methyl-CpG binding proteins. Molec. Cell. Biol. 18: 6538-6547, 1998.

NCBI and Uniprot Product Information

• NCBI GI #: 4505117
• NCBI GeneID: 8932
• NCBI Accession #: NP_003918.1
• NCBI GenBank Nucleotide #: NM_003927.4
• UniProt Accession #: Q9UBB5; O95242; Q9UIS8
• Molecular Weight: 31,745 Da
• NCBI Official Full Name: methyl-CpG-binding domain protein 2 isoform 1
• NCBI Official Synonym Full Names: methyl-CpG binding domain protein 2
• NCBI Official Symbol: MBD2
• NCBI Official Synonym Symbols: DMTase; NY-CO-41
• NCBI Protein Information: methyl-CpG-binding domain protein 2
• UniProt Protein Name: Methyl-CpG-binding domain protein 2
• Protein Family: Methyl-CpG-binding domain protein
• UniProt Gene Name: MBD2
• UniProt Synonym Gene Names: DMTase
• UniProt Entry Name: MBD2_HUMAN

NCBI Description

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

MBD2: Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. May enhance the activation of some unmethylated cAMP-responsive promoters. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 18q21

Cellular Component: cytoplasm; heterochromatin; histone deacetylase complex; nuclear chromatin; nucleoplasm; nucleus; protein complex

Molecular Function: methyl-CpG binding; mRNA binding; nucleosomal DNA binding; protein binding; protein domain specific binding; satellite DNA binding; siRNA binding

Biological Process: ATP-dependent chromatin remodeling; cellular protein complex assembly; chromatin silencing at rDNA; maternal behavior; methylation-dependent chromatin silencing; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of Wnt receptor signaling pathway; regulation of cell proliferation; transcription, DNA-dependent