Western Blot (WB)
(MBD2 pAb tested by Western blot. The analysis was performed using HeLa nuclear extract and MBD2 pAb at a 1:500 dilution.)
Rabbit anti-Human MBD2 Polyclonal Antibody | anti-MBD2 antibody
MBD2 antibody (pAb)
WB: 1:250-1:1,000 dilution
Western Blot (WB)
(MBD2 pAb tested by Western blot. The analysis was performed using HeLa nuclear extract and MBD2 pAb at a 1:500 dilution.)
Western Blot (WB)
(MBD2 pAb tested by Western blot. The analysis was performed using HeLa nuclear extract and MBD2 pAb at a 1:500 dilution.)
Background: MBD2 (methyl-CpG binding domain protein 2) is thought to function as a mediator of the biological consequences of DNA methylation. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as development and control of gene expression. It is generally associated with the repressive chromatin state. The complex series of events leading to this repressive state involve the coordinated regulation of DNA methyltransferases and two other groups of proteins called the Methyl-CpG binding proteins (MBD proteins) and the Kaiso family of proteins. The MBD family of proteins include MeCP2, MBD1, MBD2, MBD3 and MBD4. MBD2 is a transcriptional repressor which is associated with histone deacetylases (HDAC) in the Sin3 and the MeCP1 complexes in mammalian cells. The HDAC complexes, also containing MBD3 and MeCP2, are involved in packaging the genomic DNA into inactive chromatin.
NCBI and Uniprot Product Information
Uniprot Description
MBD2: Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. May enhance the activation of some unmethylated cAMP-responsive promoters. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 18q21
Cellular Component: nucleoplasm; heterochromatin; protein complex; cytoplasm; nuclear chromatin; histone deacetylase complex; nucleus
Molecular Function: mRNA binding; protein domain specific binding; protein binding; nucleosomal DNA binding; siRNA binding; methyl-CpG binding; satellite DNA binding
Biological Process: cellular protein complex assembly; transcription, DNA-dependent; maternal behavior; negative regulation of gene expression, epigenetic; ATP-dependent chromatin remodeling; gene expression; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of Wnt receptor signaling pathway; regulation of gene expression, epigenetic; regulation of cell proliferation
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Product Notes
The MBD2 mbd2 (Catalog #AAA388485) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MBD2 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MBD2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1:250-1:1,000 dilution. Researchers should empirically determine the suitability of the MBD2 mbd2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MBD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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