Human Translationally-controlled tumor protein ELISA Kit | TPT1 elisa kit

Human Translationally-controlled tumor protein ELISA Kit

Cat. Num. AAA763241

• Gene Names: TPT1; HRF; p02; p23; TCTP
• Reactivity: Human
• Synonyms: Translationally-controlled tumor protein; Human Translationally-controlled tumor protein ELISA Kit; TPT1/Translationally-controlled Tumor Protein 1/TCTP/Fortilin/HRF/Histamine-releasing factor/p23/translationally-controlled tumor protein/tumor protein; translationally-controlled 1; TPT1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TPT1. No significant cross-reactivity or interference between TPT1 and analogues was observed.
• Sequence Length: 197

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Intra-assay Precision: Intra-Assay: CV<8%
• Inter-assay Precision: Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TPT1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 555943903
• NCBI GeneID: 7178
• NCBI Accession #: NP_001273201.1
• NCBI GenBank Nucleotide #: NM_001286272.1
• Molecular Weight: 15,597 Da
• NCBI Official Full Name: translationally-controlled tumor protein isoform 1
• NCBI Official Synonym Full Names: tumor protein, translationally-controlled 1
• NCBI Official Symbol: TPT1
• NCBI Official Synonym Symbols: HRF; p02; p23; TCTP
• NCBI Protein Information: translationally-controlled tumor protein
• UniProt Protein Name: Translationally-controlled tumor protein
• Protein Family: tRNA 2'-phosphotransferase
• UniProt Gene Name: TPT1
• UniProt Synonym Gene Names: TCTP; HRF
• UniProt Entry Name: TCTP_HUMAN

Uniprot Description

TPT1: a novel heat shock protein with antiapoptotic and chaperone-like activity. Its levels are highly regulated in response to various cellular stimuli and stresses. Downregulated in response to proapoptotic treatments that induce Ca(++) stress, in a PKR-dependent manner. Colocalizes with CHFR to the mitotic spindle. Appears to be involved in calcium binding and microtubule stabilization. Its exosomal secretion is induced following DNA damage. Interacts with MCL1 and STEAP3, a p53-inducible transmembrane protein that facilitates the exosomal secretion of TPT1. Secreted TPT1 acts as a histamine-releasing factor, participates in inflammatory responses by inducing the release of histamine, and may play a role in tumor reversion. May play a direct role in placental calcium transport. Phosphorylation by Plk may decrease its microtubule-stabilizing activity and promote microtubule dynamics following metaphase. Critical for peripheral T cell maintenance and TCR-mediated cell proliferation.

Protein type: Microtubule-binding; Calcium-binding

Chromosomal Location of Human Ortholog: 13q14

Cellular Component: cytoplasm; extracellular space; multivesicular body; nucleus; tubulin complex

Molecular Function: calcium ion binding; protein binding

Biological Process: calcium ion transport; cellular calcium ion homeostasis; negative regulation of apoptosis; regulation of apoptosis; response to virus