Western Blot (WB)
(Western Blot: Sample: Lane1: Human Jurkat Cells; Lane2: Human HepG2 Cells; Lane3: Human Raji Cells; Lane4: Rat Brain Tissue.)
Rabbit anti-Rat Matrix Metalloproteinase 9 (MMP9) Polyclonal Antibody | anti-MMP9 antibody
Polyclonal Antibody to Matrix Metalloproteinase 9 (MMP9)
MGHHHHHHSGSEF-ANGAP CHFPFTFEGR SYLSCTTDGR NDGKPWCGTT ADYDTDRKYG FCPSENLYTE HGNGDGKPCV FPFIFEGHSY SACTTKGRSD GYRWCATTAN YDQDKADGFC PTRADVTVTG GNSAGEMCVF PFVFLGKQYS TCTSEGRSDG RLWCATTSNF DADKKWGFCP D
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Jurkat Cells; Lane2: Human HepG2 Cells; Lane3: Human Raji Cells; Lane4: Rat Brain Tissue.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Jurkat Cells; Lane2: Human HepG2 Cells; Lane3: Human Raji Cells; Lane4: Rat Brain Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant MMP9, Rat.)
Western Blot (WB)
(Western Blot: Sample: Recombinant MMP9, Rat.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Spleen Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Spleen Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Kidney Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Kidney Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Intestine Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Intestine Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Liver Tissue)
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Jurkat cell lysate;;Lane 2: MMP9 knockout Jurkat cell lysate;;Predicted MW: 79kDa ;Observed MW: 62kDa;Primary Ab: 2ug/ml Rabbit Anti-Rat MMP9 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Jurkat cell lysate;;Lane 2: MMP9 knockout Jurkat cell lysate;;Predicted MW: 79kDa ;Observed MW: 62kDa;Primary Ab: 2ug/ml Rabbit Anti-Rat MMP9 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047))
NCBI and Uniprot Product Information
Uniprot Description
LCN2: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. Belongs to the calycin superfamily. Lipocalin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 2 B|2 22.09 cM
Cellular Component: cytosol; extracellular region; extracellular space
Molecular Function: iron ion binding; protease binding; protein binding; protein homodimerization activity; transporter activity
Biological Process: apoptosis; immune system process; innate immune response; ion transport; iron ion homeostasis; positive regulation of cell projection organization; response to drug; response to herbicide; response to virus; siderophore transport; transport
Research Articles on MMP9
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Product Notes
The MMP9 lcn2 (Catalog #AAA2006863) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Matrix Metalloproteinase 9 (MMP9) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Matrix Metalloproteinase 9 (MMP9) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the MMP9 lcn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with a His-tag and its sequence is listed below. MGHHHHHHSG SEF-ANGAP CHFPFTFEGR SYLSCTTDGR NDGKPWCGTT ADYDTDRKYG FCPSENLYTE HGNGDGKPCV FPFIFEGHSY SACTTKGRSD GYRWCATTAN YDQDKADGFC PTRADVTVTG GNSAGEMCVF PFVFLGKQYS TCTSEGRSDG RLWCATTSNF DADKKWGFCP D. It is sometimes possible for the material contained within the vial of "Matrix Metalloproteinase 9 (MMP9), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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