Human MTPN (Myotrophin) ELISA Kit | MTPN elisa kit

Human MTPN (Myotrophin) ELISA Kit

Cat. Num. AAA7612155

• Gene Names: MTPN; V-1; GCDP
• Reactivity: Human
• Synonyms: MTPN (Myotrophin); Human MTPN (Myotrophin) ELISA Kit; Myotrophin; Protein V-1; MTPN; MTPN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MTPN. No significant cross-reactivity or interference between MTPN and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MTPN elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 21956645
• NCBI GeneID: 136319
• NCBI Accession #: NP_665807.1
• NCBI GenBank Nucleotide #: NM_145808.3
• UniProt Accession #: P58546
• Molecular Weight: 12,895 Da
• NCBI Official Full Name: myotrophin
• NCBI Official Synonym Full Names: myotrophin
• NCBI Official Symbol: MTPN
• NCBI Official Synonym Symbols: V-1; GCDP
• NCBI Protein Information: myotrophin; granule cell differentiation protein; protein V-1
• UniProt Protein Name: Myotrophin
• Protein Family: Myotrophin
• UniProt Gene Name: MTPN
• UniProt Entry Name: MTPN_HUMAN

NCBI Description

The transcript produced from this gene is bi-cistronic and can encode both myotrophin and leucine zipper protein 6. The myotrophin protein is associated with cardiac hypertrophy, where it is involved in the conversion of NFkappa B p50-p65 heterodimers to p50-p50 and p65-p65 homodimers. This protein also has a potential function in cerebellar morphogenesis, and it may be involved in the differentiation of cerebellar neurons, particularly of granule cells. A cryptic ORF at the 3' end of this transcript uses a novel internal ribosome entry site and a non-AUG translation initiation codon to produce leucine zipper protein 6, a 6.4 kDa tumor antigen that is associated with myeloproliferative disease. [provided by RefSeq, Jul 2008]

Uniprot Description

MTPN: Promotes dimerization of NF-kappa-B subunits and regulates NF-kappa-B transcription factor activity. Plays a role in the regulation of the growth of actin filaments. Inhibits the activity of the F-actin-capping protein complex formed by the CAPZA1 and CAPZB heterodimer. Promotes growth of cardiomyocytes, but not cardiomyocyte proliferation. Promotes cardiac muscle hypertrophy. Belongs to the myotrophin family.

Chromosomal Location of Human Ortholog: 7q33

Cellular Component: F-actin capping protein complex; axon; perinuclear region of cytoplasm; cytosol; nucleus

Biological Process: regulation of translation; neuron differentiation; catecholamine metabolic process; skeletal muscle regeneration; striated muscle cell differentiation; cell growth; positive regulation of protein metabolic process; positive regulation of cell growth; cerebellar granule cell differentiation; activation of NF-kappaB transcription factor; regulation of striated muscle development