Rabbit anti-Mouse Matrix Metalloproteinase 12 (MMP12) Polyclonal Antibody | anti-MMP12 antibody

Polyclonal Antibody to Matrix Metalloproteinase 12 (MMP12)

Cat. Num. AAA2006356

• Gene Names: Mmp12; MME; Mmel; AV378681
• Reactivity: Mouse
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Matrix Metalloproteinase 12 (MMP12); Polyclonal Antibody; Polyclonal Antibody to Matrix Metalloproteinase 12 (MMP12); anti-MMP12 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against MMP12. It has been selected for its ability to recognize MMP12 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.; MGHHHHHHSGSEF- AVTTVGE KIFFFKDWFF WWKLPGSPAT NITSISSIWP SIPSGIQAAY EIESRNQLFL FKDEKYWLIN NLVPEPHYPR SIYSLGFSAS VKKVDAAVFD PLRQKVYFFV DKHYWRYDVR QELMDPAYPK LISTHFPGIK PKIDAVLYFK RHYYIFQGAY QLEYDPLFRR VTKTLKSTSW FGC
• Sequence Length: 470

• Applicable Applications for anti-MMP12 antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant MMP12 (Ala294~Cys473) expressed in E.coli.
• Cross Reactivity: Mouse
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2042001
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant MMP12, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Lung Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Stomach Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Kidney Tissue.)

NCBI and Uniprot Product Information

• NCBI GI #: 991820331
• NCBI GeneID: 17381
• NCBI Accession #: NP_001307005.1
• NCBI GenBank Nucleotide #: NM_001320076.1
• UniProt Accession #: P34960; Q3TB28; Q3TBV6; Q3TBV9; Q3TD61; Q3U1S8; Q3U1S9; Q3U2R8; Q3UBC5; Q4FJM7; Q8BJ92; Q8BJB3
• Molecular Weight: 54,971 Da
• NCBI Official Full Name: macrophage metalloelastase isoform 2
• NCBI Official Synonym Full Names: matrix metallopeptidase 12
• NCBI Official Symbol: Mmp12
• NCBI Official Synonym Symbols: MME; Mmel; AV378681
• NCBI Protein Information: macrophage metalloelastase
• UniProt Protein Name: Macrophage metalloelastase
• Protein Family: Macrophage metalloelastase
• UniProt Gene Name: Mmp12
• UniProt Synonym Gene Names: Mme; Mmel; MME; MMP-12

NCBI Description

This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme. Mice lacking the encoded protein have a diminished capacity to degrade extracellular matrix components, do not develop emphysema in response to long-term exposure to cigarette smoke, and exhibit impaired clearance and increased mortality upon bacterial infection. This gene is located in a cluster of other matrix metalloproteinase genes on chromosome 9. Alternate splicing generates multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2016]

Uniprot Description

MMP12: May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. Belongs to the peptidase M10A family.

Protein type: EC 3.4.24.65; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9 A1|9 2.46 cM

Cellular Component: cytoplasm; extracellular matrix; extracellular region; extracellular space; nucleus; proteinaceous extracellular matrix

Molecular Function: calcium ion binding; collagen binding; hydrolase activity; metal ion binding; metalloendopeptidase activity; metallopeptidase activity; peptidase activity; sequence-specific DNA binding; zinc ion binding

Biological Process: negative regulation of transcription from RNA polymerase II promoter; positive regulation of epithelial cell proliferation involved in wound healing; positive regulation of transcription from RNA polymerase II promoter; protein import into nucleus; proteolysis; regulation of defense response to virus by host; wound healing; wound healing, spreading of epidermal cells