Mouse anti-Human Hsc70 (Hsp73) Monoclonal Antibody | anti-Hsc70 antibody

Anti-Hsc70 (Hsp73) Antibody

Cat. Num. AAA4157814

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Protein G Purified
• Synonyms: Hsc70 (Hsp73); Monoclonal Antibody; Anti-Hsc70 (Hsp73) Antibody; anti-Hsc70 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Specificity: This Protein G purified monoclonal antibody reacts with human Hsc70 (Hsp73) protein. A BLAST analysis was used to suggest cross-reactivity with Hsc70 from human, bovine, mouse, rat, C.elegans, beluga, dog, chicken, Drosophila, carp, salmon, trout, guinea pig, hamster, monkey, pig, cucumber, pea, rabbit, sheep and Xenopus sources based on 100% homology with the immunizing sequence. This antibody recognizes both the inducible hsp and the constitutively expressed hsc as 72 kDa and 73 kDa proteins, respectively. Crossreactivity with Hsp70 from other sources has not been determined.
• Purity/Purification: Protein G Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)

• Applicable Applications for anti-Hsc70 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: This protein G purified antibody has been tested for use in western blotting, immunoelectron microscopy, immunohistochemistry and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a doublet band approximately 72/73 kDa in size corresponding to Hsc70 (Hsp73) by western blotting in the appropriate cell lysate or extract. In general, a 1:1000 dilution is suggested for most applications and is suitable to detect Hsc70 (Hsp73) in 20 ug of heat shocked HeLa cell lysate by western blotting.
• Immunogen: This Protein G purified monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with a synthetic peptide corresponding to a region of human Hsc70 (Hsp73) protein.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-Hsc70 antibody

Hsp70 genes encode abundant heatinducible 70-kDa hsps (hsp70s). In most eukaryotes, hsp70 genes exist as part of a multigene family. Hsp70s are found in most cellular compartments of eukaryotes, including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two-thirds of hsp70s are more conserved than the C-terminal one-third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins, preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization, and protein transport.