Western Blot (WB)
(Western blot analysis on HepG2 cell lysate using PKNOX2 Antibody, The lane on the left is treated with the antigen-specific peptide.)
Rabbit anti-Human, Mouse PKNOX2 Polyclonal Antibody | anti-PKNOX2 antibody
PKNOX2 Antibody
Gene Names
PKNOX2; PREP2
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
PKNOX2; Polyclonal Antibody; PKNOX2 Antibody; FLJ13074; Homeobox protein PKNOX2; Homeobox protein PREP 2; Homeobox protein PREP-2; PBX/knotted 1 homeobox 2; PBX/knotted homeobox 2; PKNX2_HUMAN; PREP2; anti-PKNOX2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
PKNOX2 antibody detects endogenous levels of PKNOX2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
472
Applicable Applications for anti-PKNOX2 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human PKNOX2
Subcellular Location
Nucleus.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (90%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis on HepG2 cell lysate using PKNOX2 Antibody, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis on HepG2 cell lysate using PKNOX2 Antibody, The lane on the left is treated with the antigen-specific peptide.)
Immunofluorescene (IF)
(MBS9600422 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9600422 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-PKNOX2 antibody
Description: PKNOX2 Belongs to the TALE/MEIS homeobox family. Note: This description may include information from UniProtKB.
Similarity: Belongs to the TALE/MEIS homeobox family.
Similarity: Belongs to the TALE/MEIS homeobox family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 55 kDa
Predicted: 53 kDa
Predicted: 53 kDa
NCBI Official Full Name
homeobox protein PKNOX2
NCBI Official Synonym Full Names
PBX/knotted 1 homeobox 2
NCBI Official Symbol
PKNOX2
NCBI Official Synonym Symbols
PREP2
NCBI Protein Information
homeobox protein PKNOX2
UniProt Protein Name
Homeobox protein PKNOX2
Protein Family
UniProt Gene Name
PKNOX2
UniProt Synonym Gene Names
PREP2
NCBI Description
Homeodomain proteins are sequence-specific transcription factors that share a highly conserved DNA-binding domain and play fundamental roles in cell proliferation, differentiation, and death. PKNOX2 belongs to the TALE (3-amino acid loop extension) class of homeodomain proteins characterized by a 3-amino acid extension between alpha helices 1 and 2 within the homeodomain (Imoto et al., 2001 [PubMed 11549286]).[supplied by OMIM, Oct 2009]
Research Articles on PKNOX2
Similar Products
Product Notes
The PKNOX2 pknox2 (Catalog #AAA9600422) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PKNOX2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PKNOX2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:2000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the PKNOX2 pknox2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKNOX2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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