Rabbit anti-Human, Mouse MARCH5 Polyclonal Antibody | anti-MARCH5 antibody

MARCH5, NT (MARCH5, RNF153, E3 ubiquitin-protein ligase MARCH5, Membrane-associated RING finger protein 5, Membrane-associated RING-CH protein V, Mitochondrial ubiquitin ligase, RING finger protein 153) (Azide free) (HRP)

Cat. Num. AAA6318972

• Gene Names: MARCH5; MITOL; RNF153; MARCH-V
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MARCH5; Polyclonal Antibody; NT (MARCH5; RNF153; E3 ubiquitin-protein ligase MARCH5; Membrane-associated RING finger protein 5; Membrane-associated RING-CH protein V; Mitochondrial ubiquitin ligase; RING finger protein 153) (Azide free) (HRP); mitochondrial ubiquitin ligase; RING finger protein 153; anti-MARCH5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-MARCH5 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: MARCH5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11-41 amino acids from the N-terminal region of human MARCH5.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MARCH5 Antibody (N-term) in mouse kidney tissue lysates (35ug/lane).MARCH5 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(MARCH5 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MARCH5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(MARCH5 Antibody (N-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-MARCH5 antibody

MARCH5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2; MIM 608507) and DRP1 (DNM1L; MIM 603850) (Nakamura et al., 2006 [PubMed 16936636]).

Product Categories/Family for anti-MARCH5 antibody

Antibodies; Ubiquitin Ligases

References

Yonashiro, R., et al. Mol. Biol. Cell 20(21):4524-4530(2009). Karbowski, M., et al. J. Cell Biol. 178(1):71-84(2007). Nakamura, N., et al. EMBO Rep. 7(10):1019-1022(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 8923415
• NCBI GeneID: 54708
• NCBI Accession #: NP_060294
• NCBI GenBank Nucleotide #: NM_017824
• UniProt Accession #: Q9NX47
• Molecular Weight: 31,232 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase MARCH5
• NCBI Official Synonym Full Names: membrane-associated ring finger (C3HC4) 5
• NCBI Official Symbol: MARCH5
• NCBI Official Synonym Symbols: MITOL; RNF153; MARCH-V
• NCBI Protein Information: E3 ubiquitin-protein ligase MARCH5; membrane-associated RING finger protein 5; membrane-associated RING-CH protein V; mitochondrial ubiquitin ligase; ring finger protein 153
• UniProt Protein Name: E3 ubiquitin-protein ligase MARCH5
• UniProt Gene Name: MARCH5
• UniProt Synonym Gene Names: RNF153; MARCH-V; MITOL
• UniProt Entry Name: MARH5_HUMAN

Uniprot Description

MARCH5: Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission. May play a role in the prevention of cell senescence acting as a regulator of mitochondrial quality control. Promotes ubiquitination of FIS1, DNM1L and MFN1.

Protein type: EC 6.3.2.-; Membrane protein, multi-pass; Ubiquitin ligase; Ligase; Membrane protein, integral; EC 6.3.2.19; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 10q23.32-q23.33

Cellular Component: mitochondrial outer membrane; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: GTPase binding; protein binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: protein autoubiquitination; protein polyubiquitination