Goat MARCH10 Polyclonal Antibody | anti-MARCH10 antibody

Goat anti-MARCH10 Antibody

Cat. Num. AAA421725

• Gene Names: MARCH10; RNF190; MARCH-X
• Reactivity: Tested: Rat; Expected from sequence similarity: Human, Rat, Cow
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: MARCH10; Polyclonal Antibody; Goat anti-MARCH10 Antibody; membrane-associated ring finger (C3HC4) 10; FLJ35757; MARCH-X; RNF190; ring finger protein 190; MARCH10 antibody; membrane-associated ring finger (C3HC4) 10 antibody; FLJ35757 antibody; MARCH-X antibody; RNF190 antibody; ring finger protein 190 antibody; anti-MARCH10 antibody

• Host: Goat
• Reactivity: Tested: Rat; Expected from sequence similarity: Human, Rat, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: SRNYPQPRTEE
• Sequence Length: 808

• Applicable Applications for anti-MARCH10 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 8000.; Western Blot: Approx.90 kDa band observed in Rat Testis lysates (calculated MW of 90.5 kDa according to Human NP_689811.2 and of 87.9 kDa according to Mouse NP_766156.2). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 37 kDa. This band was successfully blocked by incubation with the immunising peptide.
• Immunogen: Peptide with sequence C-SRNYPQPRTEE, from the internal region of the protein sequence according to NP_001094345.1; NP_689811.2.
• Epitope: Internal region
• Note: This antibody is expected to recognise both reported isoforms (NP_001094345.1; NP_689811.2), which are identical.
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.3ug/ml) staining of Rat Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 154937342
• NCBI GeneID: 162333
• NCBI Accession #: NP_001094345.1; NP_689811.2
• NCBI GenBank Nucleotide #: NM_001100875.2
• Molecular Weight: 90,511 Da
• NCBI Official Full Name: probable E3 ubiquitin-protein ligase MARCH10 isoform 1
• NCBI Official Synonym Full Names: membrane associated ring-CH-type finger 10
• NCBI Official Symbol: MARCH10
• NCBI Official Synonym Symbols: RNF190; MARCH-X
• NCBI Protein Information: probable E3 ubiquitin-protein ligase MARCH10
• UniProt Protein Name: Probable E3 ubiquitin-protein ligase MARCH10
• UniProt Gene Name: MARCH10
• UniProt Synonym Gene Names: RNF190; MARCH-X
• UniProt Entry Name: MARHA_HUMAN

NCBI Description

MARCH10 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Morokuma et al., 2007 [PubMed 17604280]).[supplied by OMIM, Apr 2010]

Uniprot Description

MARCH10: E3 ubiquitin-protein ligase (Probable). E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.

Protein type: Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.-; Ligase; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 17q23.2

Molecular Function: ligase activity; protein binding; zinc ion binding

Biological Process: protein ubiquitination