Western Blot (WB)
(MARCH10 Antibody (Center) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the MARCH10 antibody detected the MARCH10 protein (arrow).)
Rabbit anti-Human MARCH10 Polyclonal Antibody | anti-MARCH10 antibody
MARCH10, ID (MARCH10, RNF190, Probable E3 ubiquitin-protein ligase MARCH10, Membrane-associated RING finger protein 10, Membrane-associated RING-CH protein X, RING finger protein 190) (Azide free) (HRP)
Gene Names
MARCH10; RNF190; MARCH-X
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
MARCH10; Polyclonal Antibody; ID (MARCH10; RNF190; Probable E3 ubiquitin-protein ligase MARCH10; Membrane-associated RING finger protein 10; Membrane-associated RING-CH protein X; RING finger protein 190) (Azide free) (HRP); RING finger protein 190; anti-MARCH10 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-MARCH10 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
MARCH10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 247-274 amino acids from the Central region of human MARCH10.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(MARCH10 Antibody (Center) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the MARCH10 antibody detected the MARCH10 protein (arrow).)
Western Blot (WB)
(MARCH10 Antibody (Center) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the MARCH10 antibody detected the MARCH10 protein (arrow).)
Related Product Information for anti-MARCH10 antibody
MARCH10 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Morokuma et al., 2007 [PubMed 17604280]).
Product Categories/Family for anti-MARCH10 antibody
References
Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010). Oguri, M., et al. Am. J. Hypertens. 23(1):70-77(2010). Yoshida, T., et al. Int. J. Mol. Med. 24(4):539-547(2009). Morokuma, Y., et al. J. Biol. Chem. 282(34):24806-24815(2007).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
90,511 Da
NCBI Official Full Name
probable E3 ubiquitin-protein ligase MARCH10 isoform 1
NCBI Official Synonym Full Names
membrane associated ring-CH-type finger 10
NCBI Official Symbol
MARCH10
NCBI Official Synonym Symbols
RNF190; MARCH-X
NCBI Protein Information
probable E3 ubiquitin-protein ligase MARCH10
UniProt Protein Name
Probable E3 ubiquitin-protein ligase MARCH10
UniProt Gene Name
MARCH10
UniProt Synonym Gene Names
RNF190; MARCH-X
NCBI Description
MARCH10 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Morokuma et al., 2007 [PubMed 17604280]).[supplied by OMIM, Apr 2010]
Uniprot Description
MARCH10: E3 ubiquitin-protein ligase (Probable). E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
Protein type: EC 6.3.2.-; EC 6.3.2.19; Ligase; Ubiquitin conjugating system; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 17q23.2
Molecular Function: protein binding; transferase activity; zinc ion binding
Biological Process: protein ubiquitination
Research Articles on MARCH10
Similar Products
Product Notes
The MARCH10 march10 (Catalog #AAA6318939) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MARCH10, ID (MARCH10, RNF190, Probable E3 ubiquitin-protein ligase MARCH10, Membrane-associated RING finger protein 10, Membrane-associated RING-CH protein X, RING finger protein 190) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MARCH10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MARCH10 march10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MARCH10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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