Mouse anti-Human IMPDH2 Monoclonal Antibody | anti-IMPDH2 antibody

IMPDH2 (Inosine-5'-monophosphate Dehydrogenase 2, IMP Dehydrogenase 2, IMPD 2, IMPDH 2, IMPDH-II, IMPD2) (AP)

Cat. Num. AAA6131872

• Gene Names: IMPDH2; IMPD2; IMPDH-II
• Reactivity: Human
• Applications: ELISA, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: IMPDH2; Monoclonal Antibody; IMPDH2 (Inosine-5'-monophosphate Dehydrogenase 2; IMP Dehydrogenase 2; IMPD 2; IMPDH 2; IMPDH-II; IMPD2) (AP); anti-IMPDH2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1E12-B6
• Specificity: Recognizes human IMPDH2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 1648

• Applicable Applications for anti-IMPDH2 antibody: ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-514 from human IMPDH2 (AAH12840) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MADYLISGGTSYVPDDGLTAQQLFNCGDGLTYNDFLILPGYIDFTADQVDLTSALTKKITLKTPLVSSPMDTVTEAGMAIAMALTGGIGFIHHNCTPEFQANEVRKVKKYEQGFITDPVVLSPKDRVRDVFEAKARHGFCGIPITDTGRMGSRLVGIISSRDIDFLKEEEHDCFLEEIMTKREDLVVAPAGITLKEANEILQRSKKGKLPIVNEDDELVAIIARTDLKKNRDYPLASKDAKKQLLCGAAIGTHEDDKYRLDLLAQAGVDVVVLDSSQGNSIFQINMIKYIKDKYPNLQVIGGNVVTAAQAKNLIDAGVDALRVGMGSGSICITQEVLACGRPQATAVYKVSEYARRFGVPVIADGGIQNVGHIAKALALGASTVMMGSLLAATTEAPGEYFFSDGIRLKKYRGMGSLDAMDKHLSSQNRYFSEADKIKVAQGVSGAVQDKGSIHKFVPYLIAGIQHSCQDIGAKSLTQVRAMMYSGELKFEKRTSSAQVEGGVHSLHSYEKRLF
• Conjugate: AP
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of IMPDH2 expression in transfected 293T cell line by IMPDH2 monoclonal antibody. Lane 1: IMPDH2 transfected lysate (55.8kD). Lane 2: Non-transfected lysate.)

Immunoprecipitation (IP)

(Immunoprecipitation of IMPDH2 transfected lysate using IMPDH2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IMPDH2 rabbit polyclonal antibody.)

Testing Data

(Detection limit for recombinant GST tagged IMPDH2 is ~0.3ng/ml as a capture antibody.)

Western Blot (WB)

(Western blot analysis of IMPDH2 over-expressed 293 cell line, cotransfected with IMPDH2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with IMPDH2 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

Related Product Information for anti-IMPDH2 antibody

IMPDH2 belongs to the IMPDH/GMPR family. It catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. IMPDH2 is the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. Also IMPDH1 and IMPDH2 are targets for the important immunosuppressive drug, mycophenolic acid (MPA).

Product Categories/Family for anti-IMPDH2 antibody

Antibodies; Enzymes; Dehydrogenase

References

1. Proteomic analysis of the effects of the immunomodulatory mycotoxin deoxynivalenol. da Costa AN, Mijal RS, Keen JN, Findlay JB, Wild CP.Proteomics. 2011 Mar 9. doi: 10.1002/pmic.201000580.

NCBI and Uniprot Product Information

• NCBI GI #: 15277479
• NCBI GeneID: 3615
• NCBI Official Full Name: Homo sapiens IMP (inosine monophosphate) dehydrogenase 2, mRNA
• NCBI Official Synonym Full Names: inosine monophosphate dehydrogenase 2
• NCBI Official Symbol: IMPDH2
• NCBI Official Synonym Symbols: IMPD2; IMPDH-II
• NCBI Protein Information: inosine-5'-monophosphate dehydrogenase 2

NCBI Description

This gene encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. This gene is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation. [provided by RefSeq, Jul 2008]