Western Blot (WB)
(MAPRE1 Antibody (N-term) western blot analysis in mouse brain tissue lysates (15ug/lane).This demonstrates the MAPRE1 antibody detected MAPRE1 protein (arrow).)
Rabbit MAPRE1 Polyclonal Antibody | anti-MAPRE1 antibody
MAPRE1 Antibody (N-term)
IHC-P~~1:50-100
FC:1:10~50
Western Blot (WB)
(MAPRE1 Antibody (N-term) western blot analysis in mouse brain tissue lysates (15ug/lane).This demonstrates the MAPRE1 antibody detected MAPRE1 protein (arrow).)
Western Blot (WB)
(MAPRE1 Antibody (N-term) western blot analysis in mouse brain tissue lysates (15ug/lane).This demonstrates the MAPRE1 antibody detected MAPRE1 protein (arrow).)
Immunohistochemistry (IHC)
(MAPRE1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MAPRE1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(MAPRE1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MAPRE1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(MAPRE1 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(MAPRE1 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
its binding to the APC protein which is often mutated in familial
and sporadic forms of colorectal cancer. This protein localizes to
microtubules, especially the growing ends, in interphase cells.
During mitosis, the protein is associated with the centrosomes and
spindle microtubules. The protein also associates with components
of the dynactin complex and the intermediate chain of cytoplasmic
dynein. Because of these associations, it is thought that this
protein is involved in the regulation of microtubule structures and
chromosome stability. This gene is a member of the RP/EB family.
Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :
De Groot, C.O., et al. J. Biol. Chem. 285(8):5802-5814(2010)
Jiang, K., et al. EMBO Rep. 10(8):857-865(2009)
Honnappa, S., et al. Cell 138(2):366-376(2009)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family. [provided by RefSeq, Jul 2008]
Uniprot Description
EB1: a microtubule-associated protein of the RP/EB family. Binds to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. Localizes to microtubules, especially the growing ends, in interphase cells. Associates with the centrosomes and spindle microtubules during mitosis. Also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. May be involved in the regulation of microtubule structures and chromosome stability.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 20q11.1-q11.23
Cellular Component: Golgi apparatus; centrosome; microtubule; cell projection membrane; cytoplasmic microtubule; cytoplasm; cortical microtubule cytoskeleton; spindle; cytosol
Molecular Function: protein C-terminus binding; microtubule plus-end binding; protein binding
Biological Process: mitosis; cell proliferation; cell division; negative regulation of microtubule polymerization; organelle organization and biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle
Research Articles on MAPRE1
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Product Notes
The MAPRE1 mapre1 (Catalog #AAA9208185) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 44-71. The MAPRE1 Antibody (N-term) reacts with Human, mouse (Predicted Reactivity: Bovine, Chicken, Rat, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's MAPRE1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000 IHC-P~~1:50-100 FC:1:10~50. Researchers should empirically determine the suitability of the MAPRE1 mapre1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAPRE1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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