Rabbit anti-Human, Mouse COL18A1 Polyclonal Antibody | anti-COL18A1 antibody

COL18A1, ID (COL18A1, Collagen alpha-1(XVIII) chain, Endostatin) (APC)

Cat. Num. AAA6287593

• Gene Names: COL18A1; KS; KNO; KNO1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: COL18A1; Polyclonal Antibody; ID (COL18A1; Collagen alpha-1(XVIII) chain; Endostatin) (APC); Endostatin; anti-COL18A1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-COL18A1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: COL18A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1314-1343 amino acids from the Central region of human COL18A1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(COL18A1 Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the COL18A1 antibody detected the COL18A1 protein (arrow).)

Immunohistochemistry (IHC)

(COL18A1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the COL18A1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-COL18A1 antibody

COL18A1 is the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. The proteolytically produced C-terminal fragment of type XVIII collagen is endostatin, a potent antiangiogenic protein. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities, so type XVIII collagen may play an important role in retinal structure and in neural tube closure.

Product Categories/Family for anti-COL18A1 antibody

Antibodies; Collagen

References

Hamano, Y., et al. J. Am. Soc. Nephrol. 21(9):1445-1455(2010). Roberts, K.E., et al. Gastroenterology 139(1):130-139(2010). Lee, K.S., et al. Tumour Biol. 31(3):165-170(2010). Faye, C., et al. Biochem. J. 427(3):467-475(2010). Polikarpova, S.B., et al. Bull. Exp. Biol. Med. 148(4):634-637(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 110611235
• NCBI GeneID: 80781
• NCBI Accession #: NP_085059.2
• NCBI GenBank Nucleotide #: NM_030582.3
• UniProt Accession #: P39060; Q58EX6; Q6RZ39; Q6RZ40; Q6RZ41; Q8N4S4; Q8WXI5; Q96T70; Q9UK38; Q9Y6Q7; Q9Y6Q8; A8MVI4
• Molecular Weight: 178,188 Da
• NCBI Official Full Name: collagen alpha-1(XVIII) chain isoform 1
• NCBI Official Synonym Full Names: collagen, type XVIII, alpha 1
• NCBI Official Symbol: COL18A1
• NCBI Official Synonym Symbols: KS; KNO; KNO1
• NCBI Protein Information: collagen alpha-1(XVIII) chain; collagen alpha-1(XVIII) chain; endostatin; antiangiogenic agent; multi-functional protein MFP
• UniProt Protein Name: Collagen alpha-1(XVIII) chain
• Protein Family: Collagen
• UniProt Gene Name: COL18A1
• UniProt Entry Name: COIA1_HUMAN

NCBI Description

This gene encodes the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. The proteolytically produced C-terminal fragment of type XVIII collagen is endostatin, a potent antiangiogenic protein. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities, so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

COL18A1: COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube. Defects in COL18A1 are a cause of Knobloch syndrome type 1 (KNO1). An autosomal recessive disorder defined by the occurrence of high myopia, vitreoretinal degeneration with retinal detachment, macular abnormalities and occipital encephalocele. Belongs to the multiplexin collagen family. 3 isoforms of the human protein are produced by alternative promoter.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: extracellular matrix; extracellular space; collagen; endoplasmic reticulum lumen; extracellular region; basement membrane

Molecular Function: identical protein binding; protein binding; metal ion binding; structural molecule activity

Biological Process: response to drug; extracellular matrix organization and biogenesis; collagen catabolic process; extracellular matrix disassembly; negative regulation of cell proliferation; organ morphogenesis; visual perception; positive regulation of cell proliferation; endothelial cell morphogenesis; angiogenesis; cell adhesion; response to hydrostatic pressure; positive regulation of cell migration

Disease: Knobloch Syndrome 1