Rabbit anti-Human, Mouse MAPRE1 Polyclonal Antibody | anti-MAPRE1 antibody

MAPRE1, NT (MAPRE1, Microtubule-associated protein RP/EB family member 1, APC-binding protein EB1, End-binding protein 1) (Azide free) (HRP)

Cat. Num. AAA6318906

• Gene Names: MAPRE1; EB1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MAPRE1; Polyclonal Antibody; NT (MAPRE1; Microtubule-associated protein RP/EB family member 1; APC-binding protein EB1; End-binding protein 1) (Azide free) (HRP); End-binding protein 1; anti-MAPRE1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-MAPRE1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: MAPRE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-79 amino acids from the N-terminal region of human MAPRE1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MAPRE1 Antibody (N-term) western blot analysis in mouse brain tissue lysates (15ug/lane).This demonstrates the MAPRE1 antibody detected MAPRE1 protein (arrow).)

Immunohistochemistry (IHC)

(MAPRE1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MAPRE1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(MAPRE1 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-MAPRE1 antibody

The protein encoded by this gene was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family.

Product Categories/Family for anti-MAPRE1 antibody

Antibodies; Binding Proteins

References

Jaulin, F., et al. J. Cell Biol. 190(3):443-460(2010). Olson, J.E., et al. Breast Cancer Res. Treat. (2010). In press: De Groot, C.O., et al. J. Biol. Chem. 285(8):5802-5814(2010). Jiang, K., et al. EMBO Rep. 10(8):857-865(2009). Honnappa, S., et al. Cell 138(2):366-376(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 6912494
• NCBI GeneID: 22919
• NCBI Accession #: NP_036457.1
• NCBI GenBank Nucleotide #: NM_012325.2
• UniProt Accession #: Q15691; Q3KQS8; B2R6I7; E1P5M8
• Molecular Weight: 29,999 Da
• NCBI Official Full Name: microtubule-associated protein RP/EB family member 1
• NCBI Official Synonym Full Names: microtubule-associated protein, RP/EB family, member 1
• NCBI Official Symbol: MAPRE1
• NCBI Official Synonym Symbols: EB1
• NCBI Protein Information: microtubule-associated protein RP/EB family member 1; APC-binding protein EB1; adenomatous polyposis coli-binding protein EB1; end-binding protein 1
• UniProt Protein Name: Microtubule-associated protein RP/EB family member 1
• Protein Family: Microtubule-associated protein RP/EB family
• UniProt Gene Name: MAPRE1
• UniProt Synonym Gene Names: EB1
• UniProt Entry Name: MARE1_HUMAN

NCBI Description

The protein encoded by this gene was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family. [provided by RefSeq, Jul 2008]

Uniprot Description

EB1: a microtubule-associated protein of the RP/EB family. Binds to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. Localizes to microtubules, especially the growing ends, in interphase cells. Associates with the centrosomes and spindle microtubules during mitosis. Also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. May be involved in the regulation of microtubule structures and chromosome stability.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20q11.1-q11.23

Cellular Component: Golgi apparatus; centrosome; microtubule; cell projection membrane; cytoplasmic microtubule; cytoplasm; cortical microtubule cytoskeleton; spindle; cytosol

Molecular Function: protein C-terminus binding; microtubule plus-end binding; protein binding

Biological Process: mitosis; cell proliferation; cell division; organelle organization and biogenesis; negative regulation of microtubule polymerization; mitotic cell cycle; G2/M transition of mitotic cell cycle