Human Ataxin 1 (ATXN1) ELISA Kit | ATXN1 elisa kit

Human Ataxin 1 (ATXN1) ELISA Kit

Cat. Num. AAA452766

• Gene Names: ATXN1; ATX1; SCA1; D6S504E
• Reactivity: Human
• Synonyms: Ataxin 1 (ATXN1); Human Ataxin 1 (ATXN1) ELISA Kit; Ataxin-1; ATX1; SCA1; Spinocerebellar ataxia type 1 protein; ATXN1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATXN1. No significant cross-reactivity or interference between ATXN1 and analogues was observed.
• Sequence Length: 815

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.089ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATXN1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATXN1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATXN1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ATXN1 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATXN1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ATXN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATXN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ATXN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 51479158
• NCBI GeneID: 6310
• NCBI Accession #: NP_000323.2
• NCBI GenBank Nucleotide #: NM_000332.3
• UniProt Accession #: P54253; Q17S02; Q9UJG2; Q9Y4J1
• Molecular Weight: 86,923 Da
• NCBI Official Full Name: ataxin-1
• NCBI Official Synonym Full Names: ataxin 1
• NCBI Official Symbol: ATXN1
• NCBI Official Synonym Symbols: ATX1; SCA1; D6S504E
• NCBI Protein Information: ataxin-1
• UniProt Protein Name: Ataxin-1
• Protein Family: Ataxin
• UniProt Gene Name: ATXN1
• UniProt Synonym Gene Names: ATX1; SCA1

NCBI Description

The autosomal dominant cerebellar ataxias (ADCA) are a heterogeneous group of neurodegenerative disorders characterized by progressive degeneration of the cerebellum, brain stem and spinal cord. Clinically, ADCA has been divided into three groups: ADCA types I-III. ADCAI is genetically heterogeneous, with five genetic loci, designated spinocerebellar ataxia (SCA) 1, 2, 3, 4 and 6, being assigned to five different chromosomes. ADCAII, which always presents with retinal degeneration (SCA7), and ADCAIII often referred to as the `pure' cerebellar syndrome (SCA5), are most likely homogeneous disorders. Several SCA genes have been cloned and shown to contain CAG repeats in their coding regions. ADCA is caused by the expansion of the CAG repeats, producing an elongated polyglutamine tract in the corresponding protein. The expanded repeats are variable in size and unstable, usually increasing in size when transmitted to successive generations. The function of the ataxins is not known. This locus has been mapped to chromosome 6, and it has been determined that the diseased allele contains 40-83 CAG repeats, compared to 6-39 in the normal allele, and is associated with spinocerebellar ataxia type 1 (SCA1). At least two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2016]

Uniprot Description

Chromatin-binding factor that repress Notch signaling in the absence of Notch intracellular domain by acting as a CBF1 corepressor. Binds to the HEY promoter and might assist, along with NCOR2, RBPJ-mediated repression. Binds RNA in vitro. May be involved in RNA metabolism.