Rabbit anti-Mouse Mapk3 Polyclonal Antibody | anti-Mapk3 antibody

Mapk3, CT (Mapk3, Erk1, Prkm3, Mitogen-activated protein kinase 3, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, MNK1, Microtubule-associated protein 2 kinase, p44-ERK1) (AP)

Cat. Num. AAA6318825

• Gene Names: Mapk3; p44; Erk1; Ert2; Mnk1; Erk-1; Esrk1; Prkm3; Mtap2k; p44erk1; p44mapk
• Reactivity: Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Mapk3; Polyclonal Antibody; CT (Mapk3; Erk1; Prkm3; Mitogen-activated protein kinase 3; ERT2; Extracellular signal-regulated kinase 1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; MNK1; Microtubule-associated protein 2 kinase; p44-ERK1) (AP); p44-ERK1; anti-Mapk3 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-Mapk3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: Mouse Mapk3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 352-380 amino acids from the C-terminal region of Mouse Mapk3.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Mapk3 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Mapk3 antibody detected the Mapk3 protein (arrow).)

Immunohistochemistry (IHC)

(Mouse Mapk3 Antibody (C-term) (AP14266b)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Mapk3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-Mapk3 antibody

Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2) and heat shock factor protein 4 (HSF4) (By similarity). Phosphorylates SPZ1.

Product Categories/Family for anti-Mapk3 antibody

Antibodies; Protein Kinases

References

Chen, W., et al. Biochem. Biophys. Res. Commun. 401(3):339-343(2010). Chandrakesan, P., et al. J. Biol. Chem. 285(43):33485-33498(2010). Alter, B.J., et al. J. Neurosci. 30(34):11537-11547(2010). Spruce, T., et al. Dev. Cell 19(2):207-219(2010). Bremer, J., et al. PLoS ONE 5 (8), E12450 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 21489933
• NCBI GeneID: 26417
• NCBI Accession #: NP_036082.1
• NCBI GenBank Nucleotide #: NM_011952.2
• UniProt Accession #: Q63844; Q61531; Q8K0X5; Q91YW5
• Molecular Weight: 42-44
• NCBI Official Full Name: mitogen-activated protein kinase 3
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 3
• NCBI Official Symbol: Mapk3
• NCBI Official Synonym Symbols: p44; Erk1; Ert2; Mnk1; Erk-1; Esrk1; Prkm3; Mtap2k; p44erk1; p44mapk
• NCBI Protein Information: mitogen-activated protein kinase 3; MAP kinase 3; p44 MAP kinase; pp42/MAP kinase; insulin-stimulated MAP2 kinase; extracellular signal-regulated kinase 1; microtubule-associated protein 2 kinase
• UniProt Protein Name: Mitogen-activated protein kinase 3
• UniProt Gene Name: Mapk3
• UniProt Synonym Gene Names: Erk1; Prkm3; MAP kinase 3; MAPK 3; ERK-1; p44-MAPK
• UniProt Entry Name: MK03_MOUSE

Uniprot Description

Function: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Ref.11 Ref.12

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium

By similarity.

Enzyme regulation: Phosphorylated by MAP2K1/MEK1 and MAP2K2/MEK2 on Thr-203 and Tyr-205 in response to external stimuli like insulin or NGF. Both phosphorylations are required for activity. This phosphorylation causes dramatic conformational changes, which enable full activation and interaction of MAPK1/ERK2 with its substrates. Dephosphorylated and inactivated by DUSP3, DUSP6 and DUSP9.

Subunit structure: Binds both upstream activators and downstream substrates in multimolecular complexes. Found in a complex with at least BRAF, HRAS1, MAP2K1/MEK1, MAPK3 and RGS14. Interacts with TPR

By similarity. Interacts with ADAM15, ARRB2, CANX, DAPK1 (via death domain), HSF4, IER3, MAP2K1/MEK1, NISCH, and SGK1

By similarity. Interacts with MORG1, PEA15 and MKNK2. MKNK2 isoform 1 binding prevents from dephosphorylation and inactivation. Ref.11 Ref.13 Ref.15

Subcellular location: Cytoplasm. Nucleus. Note: Autophosphorylation at Thr-207 promotes nuclear localization

By similarity. PEA15-binding redirects the biological outcome of MAPK3 kinase-signaling by sequestering MAPK3 into the cytoplasm. Ref.11 Ref.12

Domain: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification: Dually phosphorylated on Thr-203 and Tyr-205, which activates the enzyme. Ligand-activated ALK induces tyrosine phosphorylation

By similarity. Dephosphorylated by PTPRJ at Tyr-205

By similarity. Autophosphorylated on threonine and tyrosine residues in vitro. Phosphorylated upon FLT3 and KIT signaling

By similarity. Ref.9 Ref.10 Ref.14 Ref.20

Sequence similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.Contains 1 protein kinase domain.