Human Neurotrophin-3 ELISA Kit | NTF3 elisa kit

Human Neurotrophin-3 PicoKine ELISA Kit

Cat. Num. AAA175909

• Gene Names: NTF3; NT3; HDNF; NGF2; NT-3; NGF-2
• Reactivity: Human
• Synonyms: Neurotrophin-3; Human Neurotrophin-3 PicoKine ELISA Kit; neurotrophin 3; AI316846; AI835689; HDNF; MGC129711; Nerve growth factor 2; Neurotrophic factor; Neurotrophin 3 (HDNF/NT 3); Neurotrophin 3 precursor; Neurotrophin3; NGF 2; NGF2; NT3; NTF 3; NTF3; NTF3 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human NTF3
• Sequence Length: 257

• Samples: Cell culture supernates and serum
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <2pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess interassay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NTF3 elisa kit

Principle of the Assay: The Picokine™ Human NTF3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human NTF3 with a 96-well strip plate that is pre-coated with antibody specific for NTF3. The detection antibody is a biotinylated antibody specific for NTF3. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human NTF3 with immunogen: Expression system for standard: sf21; Immunogen sequence: Y139-T257. The kit is analytically validated with ready to use reagents. To measure Human NTF3, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human NTF3 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human NTF3 in the sample.!!Background/Introduction: Neurotrophin-3(NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3(Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest(NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.1

NCBI and Uniprot Product Information

• NCBI GI #: 128581
• NCBI GeneID: 4908
• UniProt Accession #: P20783; Q6FH50; B7Z1T5
• Molecular Weight: 30,800 Da
• NCBI Official Full Name: Neurotrophin-3
• NCBI Official Synonym Full Names: neurotrophin 3
• NCBI Official Symbol: NTF3
• NCBI Official Synonym Symbols: NT3; HDNF; NGF2; NT-3; NGF-2
• NCBI Protein Information: neurotrophin-3; neurotrophic factor; nerve growth factor 2
• UniProt Protein Name: Neurotrophin-3
• Protein Family: Neurotrophin
• UniProt Gene Name: NTF3
• UniProt Synonym Gene Names: NT-3; NGF-2
• UniProt Entry Name: NTF3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. [provided by RefSeq, Jul 2008]

Uniprot Description

NT-3: Seems to promote the survival of visceral and proprioceptive sensory neurons. Belongs to the NGF-beta family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: cytoplasmic membrane-bound vesicle; extracellular region

Molecular Function: neurotrophin p75 receptor binding; growth factor activity; nerve growth factor binding; receptor binding; chemoattractant activity

Biological Process: myelination; axon guidance; mechanoreceptor differentiation; epidermis development; glial cell fate determination; activation of MAPK activity; regulation of neuron differentiation; positive regulation of receptor internalization; positive regulation of glial cell differentiation; signal transduction; enteric nervous system development; induction of positive chemotaxis; positive chemotaxis; cell-cell signaling; positive regulation of cell proliferation; negative regulation of neuron apoptosis; regulation of synaptic transmission; negative regulation of peptidyl-tyrosine phosphorylation; nervous system development; activation of protein kinase B; positive regulation of peptidyl-serine phosphorylation; peripheral nervous system development; positive regulation of peptidyl-tyrosine phosphorylation; neuromuscular synaptic transmission; nerve development; smooth muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; brain development; transmembrane receptor protein tyrosine kinase signaling pathway; neurite morphogenesis; positive regulation of cell migration