Western Blot (WB)
(Anti-MAP4K1 Antibody (S368) at 1:1000 dilution + Raji whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 91 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human MAP4K1 Polyclonal Antibody | anti-MAP4K1 antibody
MAP4K1 Antibody (S368)
Gene Names
MAP4K1; HPK1
Reactivity
Human
Applications
Western Blot, ELISA
Synonyms
MAP4K1; Polyclonal Antibody; MAP4K1 Antibody (S368); Mitogen-activated protein kinase kinase kinase kinase 1; Hematopoietic progenitor kinase; MAPK/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1; HPK1; anti-MAP4K1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Ig
Specificity
This MAP4K1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-375 amino acids from human MAP4K1.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
821
Applicable Applications for anti-MAP4K1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB:~~1:1000
Subtitle
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Tissue Location
Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine.
Antigen Region
346-375 aa
Antigen Type
Synthetic Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Anti-MAP4K1 Antibody (S368) at 1:1000 dilution + Raji whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 91 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-MAP4K1 Antibody (S368) at 1:1000 dilution + Raji whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 91 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-MAP4K1 antibody
Description: MAP4K1 or HPKI (hematopoietic progenitor kinase I) is one of these mammalian kinases that have significant sequence similarity to the Saccharomyces Cerevisiae serine/threonine kinase STE20, which relays signals from G protein coupled receptors to cytosolic MAP kinase cascades. MAP4K1 may play a role in the response to environmental stress. It appears to act upstream of the JUN N terminal pathway. It may play a role in hematopoietic lineage decisions and growth regulation.
Background: Serine/threonine-protein kinase, which may play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. Able to autophosphorylate.
Background: Serine/threonine-protein kinase, which may play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. Able to autophosphorylate.
Product Categories/Family for anti-MAP4K1 antibody
References
Hu M.C.-T., Genes Dev. 10:2251-2264 (1996). Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135 (2004). Wissing J., Mol. Cell. Proteomics 6:537-547 (2007).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
91296 Da
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase kinase 1 isoform 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase kinase 1
NCBI Official Symbol
MAP4K1
NCBI Official Synonym Symbols
HPK1
NCBI Protein Information
mitogen-activated protein kinase kinase kinase kinase 1
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase kinase 1
Protein Family
UniProt Gene Name
MAP4K1
UniProt Synonym Gene Names
HPK1; MEK kinase kinase 1; MEKKK 1
UniProt Entry Name
M4K1_HUMAN
Uniprot Description
HPK1: a protein kinase of the STE20 family. Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine. May play a role in hematopoietic lineage decisions and growth regulation. Positively regulated by PGE2 and a negative regulator of PGE2-induced FOS gene transcription.
Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, STE; EC 2.7.11.1; STE group; STE20 family; KHS subfamily
Chromosomal Location of Human Ortholog: 19q13.1-q13.4
Cellular Component: cytoplasm; membrane
Molecular Function: ATP binding; MAP kinase kinase kinase kinase activity; protein binding; protein kinase activity; protein serine/threonine kinase activity
Biological Process: activation of JNK activity; activation of MAPKKK activity; cell proliferation; peptidyl-serine phosphorylation; protein amino acid autophosphorylation; protein amino acid phosphorylation; response to stress
Research Articles on MAP4K1
Similar Products
Product Notes
The MAP4K1 map4k1 (Catalog #AAA9217162) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAP4K1 Antibody (S368) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAP4K1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB:~~1:1000. Researchers should empirically determine the suitability of the MAP4K1 map4k1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAP4K1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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