Western Blot (WB)
(Fig. Western Blot analysis of various cells using HPK1 Polyclonal Antibody diluted at 1:1000.)
Rabbit anti-Human, Mouse HPK1 Polyclonal Antibody | anti-HPK1 antibody
HPK1 Polyclonal Antibody
Gene Names
MAP4K1; HPK1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
HPK1; Polyclonal Antibody; HPK1 Polyclonal Antibody; Rabbit Anti-HPK1 Polyclonal Antibody; MAP4K1; Mitogen-activated protein kinase kinase kinase kinase 1; Hematopoietic progenitor kinase; MAPK/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1; anti-HPK1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
HPK1 Polyclonal Antibody detects endogenous levels of HPK1 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid; PBS Containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
821
Applicable Applications for anti-HPK1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
Application Notes
WB: 1:500-1:2000
ELISA: 1:10000
ELISA: 1:10000
Immunogen
Synthesized peptide derived from the internal region of human HPK1 at AA range: 340-420
Preparation and Storage
Stable for one year at -20 degree C from date of shipment.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Western Blot (WB)
(Fig. Western Blot analysis of various cells using HPK1 Polyclonal Antibody diluted at 1:1000.)
Western Blot (WB)
(Fig. Western Blot analysis of various cells using HPK1 Polyclonal Antibody diluted at 1:1000.)
Related Product Information for anti-HPK1 antibody
MAP4K1 is a Protein Coding gene. Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 is a serine/threonine-protein kinase, which may play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. Able to autophosphorylate. Among its related pathways are Prostate Cancer and B Cell Receptor Signaling Pathway (WikiPathways). Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is MAP4K3.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase kinase 1 isoform 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase kinase 1
NCBI Official Symbol
MAP4K1
NCBI Official Synonym Symbols
HPK1
NCBI Protein Information
mitogen-activated protein kinase kinase kinase kinase 1
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase kinase 1
UniProt Gene Name
MAP4K1
UniProt Synonym Gene Names
HPK1; MEK kinase kinase 1; MEKKK 1
UniProt Entry Name
M4K1_HUMAN
Uniprot Description
HPK1: a protein kinase of the STE20 family. Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine. May play a role in hematopoietic lineage decisions and growth regulation. Positively regulated by PGE2 and a negative regulator of PGE2-induced FOS gene transcription.
Protein type: EC 2.7.11.1; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; STE group; STE20 family; KHS subfamily
Chromosomal Location of Human Ortholog: 19q13.1-q13.4
Cellular Component: membrane; cytoplasm
Molecular Function: protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase kinase activity; ATP binding; protein kinase activity
Biological Process: cell proliferation; peptidyl-serine phosphorylation; protein amino acid autophosphorylation; activation of MAPKKK activity; response to stress; protein amino acid phosphorylation; activation of JNK activity
Research Articles on HPK1
1. Interactions between HPK1 and its adaptor proteins related to immunity has been discussed (Review).
Similar Products
Product Notes
The HPK1 map4k1 (Catalog #AAA9702266) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HPK1 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HPK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA). WB: 1:500-1:2000 ELISA: 1:10000. Researchers should empirically determine the suitability of the HPK1 map4k1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HPK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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