Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Dilution: WB - 1:2000Anti-MAP4K1(Y381) Antibody at 1:2000 dilution + Raji whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 91 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit anti-Human MAP4K1 Polyclonal Antibody | anti-MAP4K1 antibody

MAP4K1 (Y381) Antibody

Gene Names
MAP4K1; HPK1
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Synonyms
MAP4K1; Polyclonal Antibody; MAP4K1 (Y381) Antibody; Mitogen-activated protein kinase kinase kinase kinase 1; 2.7.11.1; Hematopoietic progenitor kinase; MAPK/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1; HPK1; anti-MAP4K1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Ig
Specificity
This MAP4K1(Y381) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between amino acids from the human region of human MAP4K1(Y381).
Purity/Purification
Purified through a protein A column, followed by peptide affinity purification.
Form/Format
Supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Length
821
Applicable Applications for anti-MAP4K1 antibody
Western Blot (WB), ELISA (EIA)
Antigen Type
Synthesized Peptide
Antigen Source
Human
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Dilution: WB - 1:2000Anti-MAP4K1(Y381) Antibody at 1:2000 dilution + Raji whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 91 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (Dilution: WB - 1:2000Anti-MAP4K1(Y381) Antibody at 1:2000 dilution + Raji whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 91 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Dilution: WB - 1:2000All lanes: Anti-MAP4K1(Y381) Antibody at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 91 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (Dilution: WB - 1:2000All lanes: Anti-MAP4K1(Y381) Antibody at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 91 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-MAP4K1 antibody
Serine/threonine-protein kinase, which may play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. Able to autophosphorylate.
Product Categories/Family for anti-MAP4K1 antibody
References
Hu M.C.-T.,et al.Genes Dev. 10:2251-2264(1996). Grimwood J.,et al.Nature 428:529-535(2004). Oppermann F.S.,et al.Mol. Cell. Proteomics 8:1751-1764(2009). Mayya V.,et al.Sci. Signal. 2:RA46-RA46(2009). Burkard T.R.,et al.BMC Syst. Biol. 5:17-17(2011).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
91296 Da
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase kinase 1 isoform 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase kinase 1
NCBI Official Symbol
MAP4K1
NCBI Official Synonym Symbols
HPK1
NCBI Protein Information
mitogen-activated protein kinase kinase kinase kinase 1
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase kinase 1
UniProt Gene Name
MAP4K1
UniProt Synonym Gene Names
HPK1; MEK kinase kinase 1; MEKKK 1
UniProt Entry Name
M4K1_HUMAN

Uniprot Description

HPK1: a protein kinase of the STE20 family. Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine. May play a role in hematopoietic lineage decisions and growth regulation. Positively regulated by PGE2 and a negative regulator of PGE2-induced FOS gene transcription.

Protein type: EC 2.7.11.1; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; STE group; STE20 family; KHS subfamily

Chromosomal Location of Human Ortholog: 19q13.1-q13.4

Cellular Component: membrane; cytoplasm

Molecular Function: protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase kinase activity; ATP binding; protein kinase activity

Biological Process: cell proliferation; peptidyl-serine phosphorylation; protein amino acid autophosphorylation; activation of MAPKKK activity; response to stress; protein amino acid phosphorylation; activation of JNK activity

Research Articles on MAP4K1

Similar Products

Product Notes

The MAP4K1 map4k1 (Catalog #AAA9231346) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAP4K1 (Y381) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAP4K1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). Researchers should empirically determine the suitability of the MAP4K1 map4k1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAP4K1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.