Western Blot (WB)
(Mouse Map2k6 Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Map2k6 antibody detected the Map2k6 protein (arrow).)
Rabbit anti-Mouse Map2k6 Polyclonal Antibody | anti-map2k6 antibody
Map2k6, NT (Map2k6, Prkmk6, Sapkk3, Dual specificity mitogen-activated protein kinase kinase 6, MAPK/ERK kinase 6, SAPKK3)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Western Blot (WB)
(Mouse Map2k6 Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Map2k6 antibody detected the Map2k6 protein (arrow).)
Western Blot (WB)
(Mouse Map2k6 Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Map2k6 antibody detected the Map2k6 protein (arrow).)
NCBI and Uniprot Product Information
Uniprot Description
Function: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinases p38 and plays an important role in the regulation of cellular responses to cytokines and all kinds of stresses. The p38 MAP kinase signal transduction pathway leads to direct activation of transcription factors. Phosphorylation by MAP2K6 asymmetrically activates p38 on one side of the blastodisc, an event which is necessary for blastomere cleavage. Ref.1
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation: Activated by dual phosphorylation on Ser-234 and Thr-238 in response to a variety of cellular stresses, including UV radiation, osmotic shock, hypoxia, inflammatory cytokines, interferon gamma (IFNG), and less often by growth factors. MAP2K6/MKK6 is activated by the majority of M3Ks
By similarity.
Subunit structure: Dimer
By similarity. Interacts (via its D domain) with its MAP kinase substrates
By similarity. Interacts (via its DVD domain) with MAP3Ks activators
By similarity.
Subcellular location: Nucleus
By similarity. Cytoplasm
By similarity. Cytoplasm › cytoskeleton
By similarity. Note: Binds to microtubules
By similarity.
Developmental stage: Expressed both maternally and zygotically. Zygotic expression continues throughout development. Ref.1
Domain: The DVD domain (residues 338-361) contains a conserved docking site and is found in the mammalian MAP kinase kinases (MAP2Ks). The DVD sites bind to their specific upstream MAP kinase kinase kinases (MAP3Ks) and are essential for activation
By similarity.The D domain (residues 30-46) contains a conserved docking site and is required for the binding to MAPK substrates
By similarity.
Post-translational modification: Weakly autophosphorylated
By similarity. Phosphorylated at Ser-234 and Thr-238 by the majority of M3Ks.
Sequence similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.Contains 1 protein kinase domain.
Caution: Was originally (Ref.1) thought to be a Map2k3 protein but sequence analysis shows closer similarity to the Map2k6 proteins.
Sequence caution: The sequence AAH71285.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
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Product Notes
The map2k6 map2k6 (Catalog #AAA648368) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Map2k6, NT (Map2k6, Prkmk6, Sapkk3, Dual specificity mitogen-activated protein kinase kinase 6, MAPK/ERK kinase 6, SAPKK3) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Map2k6 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the map2k6 map2k6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Map2k6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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