Rabbit anti-Human ARPC1A Polyclonal Antibody | anti-ARPC1A antibody

ARPC1A, CT (Actin-related Protein 2/3 Complex Subunit 1A, SOP2-like Protein, SOP2L) (AP)

Cat. Num. AAA6464066

• Gene Names: ARPC1A; Arc40; SOP2L; HEL-68; SOP2Hs; HEL-S-307
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: ARPC1A; Polyclonal Antibody; CT (Actin-related Protein 2/3 Complex Subunit 1A; SOP2-like Protein; SOP2L) (AP); anti-ARPC1A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ARPC1A.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
• Sequence Length: 370

• Applicable Applications for anti-ARPC1A antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human ARPC1A (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ARPC1A Antibody (C-term) in Hela cell line lysates (35ug/lane). ARPC1A (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ARPC1A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Immunofluorescence analysis of ARPC1A Antibody (C-term) with hela cells. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of WiDr cells using ARPC1A Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-ARPC1A antibody

Antibodies; Actin Related Proteins

References

1. Laurila, E., Genes Chromosomes Cancer 48(4), 330-339 (2009). 2. Machesky, L.M., Biochem. J. 328(PT 1), 105-112 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 22907052
• NCBI GeneID: 10552
• NCBI Accession #: NP_006400
• NCBI GenBank Nucleotide #: NP_006400.2
• UniProt Accession #: Q92747
• Molecular Weight: 41.569
• NCBI Official Full Name: actin-related protein 2/3 complex subunit 1A isoform 1
• NCBI Official Synonym Full Names: actin related protein 2/3 complex subunit 1A
• NCBI Official Symbol: ARPC1A
• NCBI Official Synonym Symbols: Arc40; SOP2L; HEL-68; SOP2Hs; HEL-S-307
• NCBI Protein Information: actin-related protein 2/3 complex subunit 1A
• UniProt Protein Name: Actin-related protein 2/3 complex subunit 1A
• Protein Family: Actin-related protein 2/3 complex
• UniProt Gene Name: ARPC1A
• UniProt Synonym Gene Names: SOP2L
• UniProt Entry Name: ARC1A_HUMAN

NCBI Description

This gene encodes one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein encoded by gene ARPC1B. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

ARPC1A: Probably functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Belongs to the WD repeat ARPC1 family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: Arp2/3 protein complex; cytosol; actin cytoskeleton

Molecular Function: actin filament binding; actin binding

Biological Process: axon guidance; ephrin receptor signaling pathway; innate immune response; actin cytoskeleton organization and biogenesis