Rabbit anti-Human, Mouse MAP1D Polyclonal Antibody | anti-METAP1D antibody

MAP1D, NT (METAP1D, MAP1D, Methionine aminopeptidase 1D, mitochondrial, Methionyl aminopeptidase type 1D, mitochondrial)

Cat. Num. AAA648295

• Gene Names: METAP1D; MAP1D; MAP 1D; Metap1l; MetAP 1D
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: MAP1D; Polyclonal Antibody; NT (METAP1D; Methionine aminopeptidase 1D; mitochondrial; Methionyl aminopeptidase type 1D; mitochondrial); Anti -MAP1D; anti-METAP1D antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-METAP1D antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: MAP1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309-335 amino acids from the N-terminal region of human MAP1D.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse spleen cell line lysates (35ug/lane) using MBS648295. This demonstrates that MBS648295 detected the MAP1D protein (arrow).)

Related Product Information for anti-METAP1D antibody

The N-terminal methionine excision pathway is an essential process in which the N-terminal methionine is removed from many proteins, thus facilitating subsequent protein modification. In mitochondria, enzymes that catalyze this reaction are celled methionine aminopeptidases (MetAps, or MAPs; EC 3.4.11.18) (Serero et al., 2003 [PubMed 14532271]).

Product Categories/Family for anti-METAP1D antibody

Antibodies; Abs to Enzymes; Protease (Proteinase; Peptidase)

NCBI and Uniprot Product Information

• NCBI GI #: 40385867
• NCBI GeneID: 254042
• NCBI Accession #: NP_954697.1
• NCBI GenBank Nucleotide #: NM_199227.1
• UniProt Accession #: Q6UB28; Q1WNX3
• Molecular Weight: 37,088 Da
• NCBI Official Full Name: methionine aminopeptidase 1D, mitochondrial
• NCBI Official Synonym Full Names: methionyl aminopeptidase type 1D (mitochondrial)
• NCBI Official Symbol: METAP1D
• NCBI Official Synonym Symbols: MAP1D; MAP 1D; Metap1l; MetAP 1D
• NCBI Protein Information: methionine aminopeptidase 1D, mitochondrial; peptidase M 1D; CDS of metAP-3 within PCR fragment; methionyl aminopeptidase type 1D, mitochondrial
• UniProt Protein Name: Methionine aminopeptidase 1D, mitochondrial
• UniProt Gene Name: METAP1D
• UniProt Synonym Gene Names: MAP1D; MAP 1D; MetAP 1D
• UniProt Entry Name: MAP12_HUMAN

NCBI Description

The N-terminal methionine excision pathway is an essential process in which the N-terminal methionine is removed from many proteins, thus facilitating subsequent protein modification. In mitochondria, enzymes that catalyze this reaction are celled methionine aminopeptidases (MetAps, or MAPs; EC 3.4.11.18) (Serero et al., 2003 [PubMed 14532271]).[supplied by OMIM, Mar 2008]

Uniprot Description

Function: Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed

By similarity. May play a role in colon tumorigenesis. Ref.2

Catalytic activity: Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides. HAMAP-Rule MF_03174

Cofactor: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site

By similarity. Ref.5

Subcellular location: Mitochondrion Ref.1.

Tissue specificity: Overexpressed in colon cancer cell lines and colon tumors as compared to normal tissues (at protein level). Ref.2

Sequence similarities: Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.

Caution: It is uncertain whether Met-1 or a Met upstream of this sequence is the initiator.

Biophysicochemical propertiesKinetic parameters:KM=573 µM for Met-pro-p-nitroanilide (at pH 8) Ref.5pH dependence:Optimum pH is 7.5-8.0.

Sequence caution: The sequence AAY55948.1 differs from that shown. Reason: Erroneous initiation.