Mouse Glypican-3 (GPC3) Monoclonal Antibody | anti-GPC3 antibody

Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker) Mouse Monoclonal Antibody

Cat. Num. AAA439477

• Gene Names: GPC3; SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2
• Reactivity: Human.; Others not known
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Synonyms: Glypican-3 (GPC3); Monoclonal Antibody; Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker) Mouse Monoclonal Antibody; DGSX; Glypican proteoglycan 3; GPC3; GTR2-2; Heparan sulphate proteoglycan; Intestinal protein OCI-5; MXR7; OCI-5; SDYS; Secreted glypican-3; SGBS1; anti-GPC3 antibody

• Host: Mouse
• Reactivity: Human.; Others not known
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: SPM595
• Specificity: Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilms tumor. In patients with HCC, GPC3 is overexpressed in neoplastic liver tissue and elevated in serum, but is undetectable in normal liver, benign liver, and the serum of healthy donors. GPC3 expression is also found to be higher in HCC liver tissue than in cirrhotic liver or liver with focal lesions such as dysplastic nodules and areas of hepatic adenoma (HA) with malignant transformation. In the context of testicular germ cell tumors, GPC3 expression is up regulated in certain histologic subtypes, specifically yolk sac tumors and choriocarcinoma. A high level of GPC3 expression is also found in some types of embryonal tumors, such as Wilms tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100% in follicular carcinoma and 70% in papillary carcinoma. Expression of GPC3 in follicular carcinoma is significantly higher than that of follicular adenoma. In contrast, GPC3 is not expressed in anaplastic carcinoma.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 603

• Applicable Applications for anti-GPC3 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml); Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Cytoplasmic
• Immunogen: A recombinant fragment containing amino acids 511-580 of human glypican-3
• Hu-Chromosome Location: Xq26.2
• Positive Control: 293T cells or Hepatocellular carcinoma
• Preparation and Storage: Antibody without azide - store at -20°C to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Hepatocellular Carcinoma stained with Glypican-3 Monoclonal Antibody (SPM595))

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Melanoma stained with Glypican-3 Monoclonal Antibody (SPM595))

References

Yan, B., et al. 2011. Expression and clinicopathologic significance of glypican 3 in hepatocellular carcinoma. Ann. Diagn. Pathol. 15: 162-169.

NCBI and Uniprot Product Information

• NCBI GI #: 257471006
• NCBI GeneID: 2719
• NCBI Accession #: NP_001158089.1
• NCBI GenBank Nucleotide #: NM_001164617.1
• UniProt Accession #: P51654; Q2L880; Q2L882; C9JLE3; G3V1R0
• Molecular Weight: 67kDa
• NCBI Official Full Name: glypican-3 isoform 1
• NCBI Official Synonym Full Names: glypican 3
• NCBI Official Symbol: GPC3
• NCBI Official Synonym Symbols: SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2
• NCBI Protein Information: glypican-3
• UniProt Protein Name: Glypican-3
• Protein Family: Glypican
• UniProt Gene Name: GPC3
• UniProt Synonym Gene Names: OCI5
• UniProt Entry Name: GPC3_HUMAN

NCBI Description

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]

Uniprot Description

Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition.