Rabbit anti-Human, Mouse MAP1D Polyclonal Antibody | anti-MAP1D antibody

MAP1D, NT (METAP1D, MAP1D, Methionine aminopeptidase 1D, mitochondrial, Methionyl aminopeptidase type 1D, mitochondrial) (APC)

Cat. Num. AAA6318298

• Gene Names: METAP1D; MAP1D; MAP 1D; Metap1l; MetAP 1D
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: MAP1D; Polyclonal Antibody; NT (METAP1D; Methionine aminopeptidase 1D; mitochondrial; Methionyl aminopeptidase type 1D; mitochondrial) (APC); METAP1D; anti-MAP1D antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MAP1D. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-MAP1D antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 306-334 in the N-terminal region of human MAP1D, UniProt Accession #Q6UB28; NP_954697.1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse spleen cell line lysates (35ug/lane) usingMBS648295. This demonstrates that MBS648295 detected the MAP1D protein (arrow).)

Related Product Information for anti-MAP1D antibody

The N-terminal methionine excision pathway is an essential process in which the N-terminal methionine is removed from many proteins, thus facilitating subsequent protein modification. In mitochondria, enzymes that catalyze this reaction are celled methionine aminopeptidases (MetAps, or MAPs; EC 3.4.11.18) (Serero et al., 2003 [PubMed 14532271]).

Product Categories/Family for anti-MAP1D antibody

Antibodies; Enzymes; Protease (Proteinase; Peptidase)

References

1. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 2. Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010). 3. Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). 4. Leszczyniecka, M., et al. Oncogene 25(24):3471-3478(2006). 5. Serero, A., et al. J. Biol. Chem. 278(52):52953-52963(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 40385867
• NCBI GeneID: 254042
• NCBI Accession #: NP_954697.1
• NCBI GenBank Nucleotide #: NM_199227.1
• UniProt Accession #: Q6UB28; Q1WNX3
• Molecular Weight: 37,088 Da
• NCBI Official Full Name: methionine aminopeptidase 1D, mitochondrial
• NCBI Official Synonym Full Names: methionyl aminopeptidase type 1D (mitochondrial)
• NCBI Official Symbol: METAP1D
• NCBI Official Synonym Symbols: MAP1D; MAP 1D; Metap1l; MetAP 1D
• NCBI Protein Information: methionine aminopeptidase 1D, mitochondrial; CDS of metAP-3 within PCR fragment; MAP 1D; metAP 1D; methionyl aminopeptidase type 1D, mitochondrial; peptidase M 1D
• UniProt Protein Name: Methionine aminopeptidase 1D, mitochondrial
• Protein Family: Methionine aminopeptidase
• UniProt Gene Name: METAP1D
• UniProt Entry Name: MAP12_HUMAN

NCBI Description

The N-terminal methionine excision pathway is an essential process in which the N-terminal methionine is removed from many proteins, thus facilitating subsequent protein modification. In mitochondria, enzymes that catalyze this reaction are celled methionine aminopeptidases (MetAps, or MAPs; EC 3.4.11.18) (Serero et al., 2003 [PubMed 14532271]).[supplied by OMIM, Mar 2008]

Uniprot Description

MAP1D: Removes the N-terminal methionine from nascent proteins. May play a role in colon tumorigenesis. Belongs to the peptidase M24A family.

Protein type: EC 3.4.11.18; Protease; Mitochondrial

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: mitochondrion

Molecular Function: metalloexopeptidase activity; metal ion binding; aminopeptidase activity

Biological Process: peptidyl-methionine modification; proteolysis; N-terminal protein amino acid modification