SIRT3 sirna

SIRT3 siRNA (Human)

Cat. Num. AAA827374

• Gene Names: SIRT3; SIR2L3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SIRT3; SIRT3 siRNA (Human); SIR2L3; NAD-dependent protein deacetylase sirtuin-3 mitochondrial; hSIRT3; Regulatory protein SIR2 homolog 3; SIR2-like protein 3; SIRT3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SIRT3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 257

• Applicable Applications for SIRT3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SIRT3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SIRT3 sirna

siRNA to inhibit SIRT3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 63054862
• NCBI GeneID: 23410
• NCBI Accession #: NP_001017524.1
• NCBI GenBank Nucleotide #: NM_001017524.2
• UniProt Accession #: Q9NTG7; Q9Y6E8; B7Z5U6
• Molecular Weight: 28,567 Da
• NCBI Official Full Name: NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform b
• NCBI Official Synonym Full Names: sirtuin 3
• NCBI Official Symbol: SIRT3
• NCBI Official Synonym Symbols: SIR2L3
• NCBI Protein Information: NAD-dependent protein deacetylase sirtuin-3, mitochondrial
• UniProt Protein Name: NAD-dependent protein deacetylase sirtuin-3, mitochondrial
• Protein Family: NAD-dependent protein deacetylase sirtuin
• UniProt Gene Name: SIRT3
• UniProt Synonym Gene Names: SIR2L3; hSIRT3
• UniProt Entry Name: SIR3_HUMAN

NCBI Description

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SIRT3: a soluble mitochondrial NAD-dependent protein deacetylase. Can deacetylate and thereby activate a central metabolic regulator in the mitochondrial matrix, glutamate dehydrogenase. Mitochondrial proteins are hyperacetylated in SIRT3-deficient mice, but not in SIRT4 or SIRT5 deficient mice. SIRT3-deficient mice have no obvious metabolic phenotype, and the process of adaptive thermogenesis is normal. Can deacetylate and activate isocitrate dehydrogenase 2, an enzyme that participates in regulation of the citric acid cycle and the regeneration of antioxidants. Inhibited by nicotinamide, but not by trichostatin A. Its unprocessed form is enzymatically inactive. It is processed by mitochondrial processing peptidase (MPP) in the mitochondrial matrix to give an enzymatically active 28 kDa product.

Protein type: Mitochondrial; EC 3.5.1.-; Deacetylase

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: mitochondrion; membrane; mitochondrial matrix

Molecular Function: protein binding; enzyme binding; zinc ion binding; NAD-dependent histone deacetylase activity (H3-K14 specific); NAD+ ADP-ribosyltransferase activity

Biological Process: mitochondrion organization and biogenesis; protein amino acid ADP-ribosylation; protein amino acid deacetylation; organelle organization and biogenesis; aerobic respiration; aging