CDK5P25 recombinant protein

CDK5P25 recombinant protein

Cat. Num. AAA515301

• Gene Names: CDK5R1; p23; p25; p35; CDK5R; NCK5A; CDK5P35; p35nck5a
• Applications: Western Blot
• Purity: >85%
• Synonyms: CDK5P25; CDK5P25 recombinant protein; p25; CDK5R1; CDK5R; NCK5A; p23; CDK5P25 Protein

• Host: Sf9 insect cells
• Purity/Purification: >85%
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Concentration: 0.2 ug/ul (varies by lot)
• Sequence Length: 3870

• Applicable Applications for CDK5P25 recombinant protein: Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: N-terminal GST
• Source Note: Recombinant human CDK5R1 (98-end) was expressed by baculovirus in Sf9 insect cells
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Stable for 1 year at -70 degree C from date of shipment.

SDS-PAGE

(The purity of CDK5P25 was determined to be >85% by densitometry. Approx. MW 49kDa.)

Product Categories/Family for CDK5P25 recombinant protein

Cell Cycle Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 34304373
• NCBI GeneID: 8851
• NCBI Accession #: NP_003876.1
• NCBI GenBank Nucleotide #: NM_003885
• UniProt Accession #: Q15078; Q5U0G3; E1P664
• Molecular Weight: 49 kDa
• NCBI Official Full Name: Homo sapiens cyclin-dependent kinase 5, regulatory subunit 1 (p35) (CDK5R1), mRNA
• NCBI Official Synonym Full Names: cyclin-dependent kinase 5, regulatory subunit 1 (p35)
• NCBI Official Symbol: CDK5R1
• NCBI Official Synonym Symbols: p23; p25; p35; CDK5R; NCK5A; CDK5P35; p35nck5a
• NCBI Protein Information: cyclin-dependent kinase 5 activator 1; CDK5 activator 1; neuronal CDK5 activator; TPKII regulatory subunit; regulatory partner for CDK5 kinase; tau protein kinase II 23kDa subunit; cyclin-dependent kinase 5 regulatory subunit 1
• UniProt Protein Name: Cyclin-dependent kinase 5 activator 1
• UniProt Gene Name: CDK5R1
• UniProt Synonym Gene Names: CDK5R; NCK5A; CDK5 activator 1; p35; p25; p23
• UniProt Entry Name: CD5R1_HUMAN

NCBI Description

The protein encoded by this gene (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling. Activator of TPKII.

Subunit structure: Heterodimer composed of a catalytic subunit CDK5 and a regulatory subunit CDK5R1 (p25) and macromolecular complex composed of at least CDK5, CDK5R1 (p35) and CDK5RAP1 or CDK5RAP2 or CDK5RAP3. Only the heterodimer shows kinase activity. Interacts with EPHA4 and NGEF; may mediate the activation of NGEF by EPHA4

By similarity. Interacts with RASGRF2. Ref.8 Ref.10

Subcellular location: Cyclin-dependent kinase 5 activator 1, p35: Cell membrane; Lipid-anchor; Cytoplasmic side

Probable. Note: In the primary cortical neurons, p35 is present in the peripheries and nerve terminals. Ref.6 Ref.10 Ref.11Cyclin-dependent kinase 5 activator 1, p25: Nucleus. Cytoplasm › perinuclear region. Note: The conversion of p35 to p25 relocalizes the protein from the cell periphery to the cytoplasm, in nuclear and perinuclear regions. In the primary cortical neurons, p25 is primarily concentrated in the cell soma and is largely absent from neurites. Ref.6 Ref.10 Ref.11

Tissue specificity: Brain and neuron specific.

Post-translational modification: The p35 form is proteolytically cleaved by calpain, giving rise to the p25 form. P35 has a 5 to 10 fold shorter half-life compared to p25. The conversion results in deregulation of the CDK5 kinase: p25/CDK5 kinase displays an increased and altered tau phosphorylation in comparison to the p35/CDK5 kinase in vivo

By similarity.Myristoylated. A proper myristoylation signal is essential for the proper distribution of p35.Ubiquitinated. Degradation of p35 by proteasome results in down-regulation of CDK5 activity. During this process, CDK5 phosphorylates p35 and induces its ubiquitination and subsequent degradation. Ref.7Phosphorylation at Ser-8 and Thr-138 by CDK5 prevents calpain-mediated proteolysis.

Miscellaneous: Cleavage of p35 to p25 may be involved in the pathogenesis of cytoskeletal abnormalities and neuronal death in neurodegenerative diseases. The p25 form accumulates in neurons in the brain of patients with Alzheimer disease, but not in normal brain. This accumulation correlates with an increase in CDK5 kinase activity. Application of amyloid beta peptide A-beta(1-42) induced the conversion of p35 to p25 in primary cortical neurons. Expression of the p25/Cdk5 complex in cultured primary neurons induces cytoskeletal disruption, morphological degeneration and apoptosis.

Sequence similarities: Belongs to the cyclin-dependent kinase 5 activator family.