Rabbit anti-Human MAP Kinase Interacting Serine/threonine Kinase 2 Polyclonal Antibody | anti-MNK2 antibody

MAP Kinase Interacting Serine/threonine Kinase 2, CT (MAP Kinase Signal-integrating Kinase 2, MAPK Signal-integrating Kinase 2, MKNK2, MNK2, GPRK7) (FITC)

Cat. Num. AAA6486630

• Gene Names: MKNK2; MNK2; GPRK7
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: MAP Kinase Interacting Serine/threonine Kinase 2; Polyclonal Antibody; CT (MAP Kinase Signal-integrating Kinase 2; MAPK Signal-integrating Kinase 2; MKNK2; MNK2; GPRK7) (FITC); MAP Kinase-interacting Serine/threonine-protein Kinase 2; EC=2.7.11.1; anti-MNK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MKNK2.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-MNK2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human MKNK2 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MNK2 (MKNK2) antibody (C-term) in 293 cell line lysates (35ug/lane). MNK2 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-MNK2 antibody

Antibodies; Protein Kinases

References

1. Scheper, G.C., et al., Mol. Cell. Biol. 23(16):5692-5705 (2003). 2. Knauf, U., et al., Mol. Cell. Biol. 21(16):5500-5511 (2001). 3. Scheper, G.C., et al., Mol. Cell. Biol. 21(3):743-754 (2001). 4. Slentz-Kesler, K., et al., Genomics 69(1):63-71 (2000). 5. Haribabu, B., et al., Proc. Natl. Acad. Sci. USA 90(20):9398-9402 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 40018631
• NCBI GeneID: 2872
• NCBI Accession #: NP_951009
• NCBI GenBank Nucleotide #: NM_199054
• UniProt Accession #: Q9HBH9
• Molecular Weight: 51kDa
• NCBI Official Full Name: MAP kinase-interacting serine/threonine-protein kinase 2 isoform 1
• NCBI Official Synonym Full Names: MAP kinase interacting serine/threonine kinase 2
• NCBI Official Symbol: MKNK2
• NCBI Official Synonym Symbols: MNK2; GPRK7
• NCBI Protein Information: MAP kinase-interacting serine/threonine-protein kinase 2
• UniProt Protein Name: MAP kinase-interacting serine/threonine-protein kinase 2
• UniProt Gene Name: MKNK2
• UniProt Synonym Gene Names: GPRK7; MNK2; MAPK signal-integrating kinase 2; Mnk2
• UniProt Entry Name: MKNK2_HUMAN

NCBI Description

This gene encodes a member of the calcium/calmodulin-dependent protein kinases (CAMK) Ser/Thr protein kinase family, which belongs to the protein kinase superfamily. This protein contains conserved DLG (asp-leu-gly) and ENIL (glu-asn-ile-leu) motifs, and an N-terminal polybasic region which binds importin A and the translation factor scaffold protein eukaryotic initiation factor 4G (eIF4G). This protein is one of the downstream kinases activated by mitogen-activated protein (MAP) kinases. It phosphorylates the eukaryotic initiation factor 4E (eIF4E), thus playing important roles in the initiation of mRNA translation, oncogenic transformation and malignant cell proliferation. In addition to eIF4E, this protein also interacts with von Hippel-Lindau tumor suppressor (VHL), ring-box 1 (Rbx1) and Cullin2 (Cul2), which are all components of the CBC(VHL) ubiquitin ligase E3 complex. Multiple alternatively spliced transcript variants have been found, but the full-length nature and biological activity of only two variants are determined. These two variants encode distinct isoforms which differ in activity and regulation, and in subcellular localization. [provided by RefSeq, Aug 2011]

Uniprot Description

Mnk2: a ubiquituous CAM kinase of the MAPKKAPK family. Regulates translation by phosphorylating S209 of eIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap. The phosphorylation of eIF4E S209 by the MNK1/2 kinases is required for eIF4E's oncogenic action. Phosphorylates Spry2 on S112 and S121, leading to the ubiquitination and degradation of Spry2. Five splice-variant isoforms have been described.

Protein type: Translation; EC 2.7.11.1; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; CAMK group; MAPKAPK family; MNK subfamily

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; PML body; cytoplasm; nucleolus; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; ATP binding

Biological Process: regulation of translation; cell surface receptor linked signal transduction; hemopoiesis; protein amino acid phosphorylation