Rabbit anti-Human Cystatin A Polyclonal Antibody | anti-CSTA antibody

Anti-Cystatin A antibody

Cat. Num. AAA176853

• Gene Names: CSTA; AREI; STF1; STFA
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: Cystatin A; Polyclonal Antibody; Anti-Cystatin A antibody; Cystatin-A; cystatin A(stefin A); AREI antibody; CSTA antibody; CSTA protein antibody; Cystatin A antibody; Cystatin AS antibody; Cystatin-A antibody; Cystatin-AS antibody; CystatinA antibody; CYTA_HUMAN antibody; Stefin A antibody; Stefin A1 antibody; Stefin-A antibody; StefinA antibody; STF 1 antibody; STF1 antibody; STFA antibody; anti-CSTA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 98

• Applicable Applications for anti-CSTA antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Cystatin A (77-90aa NEDLVLTGYQVDKN).
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-Cystatin A antibody, MBS176853, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant Human CSTA, 45.0KD(M1-F98+unrelated protein,311aa)Lane 1: Recombinant Human CSTA Protein 10ngLane 2: Recombinant Human CSTA Protein 5ngLane 3: Recombinant Human CSTA Protein 2.5ngLane 4: Recombinant Human CSTA Protein 1.25ng)

Immunohistochemistry (IHC)

(Anti-Cystatin A antibody, MBS176853, IHC(P)IHC(P): Human Tonsil Tissue )

Related Product Information for anti-CSTA antibody

Description: Rabbit IgG polyclonal antibody for Cystatin-A(CSTA) detection. Tested with WB, IHC-P in Human.
Background: Cystatin A also called Stefin A is a protein that in humans is encoded by the CSTA gene. Cystatin A is a cysteine proteinase inhibitor that belongs to family 1 of the cystatin superpamily. This gene was assigned to human chromosome 3q21.1. This is an intracellular thiol proteinase inhibitor. Cystatin A plays an important role in desmosome-mediated cell-cell adhesion in the lower levels of the epidermis. Stefins have been proposed as prognostic tools for cancer.

NCBI and Uniprot Product Information

• NCBI GI #: 118177
• NCBI GeneID: 1475
• UniProt Accession #: P01040; Q6IB90
• Molecular Weight: 11,006 Da
• NCBI Official Full Name: Cystatin-A
• NCBI Official Synonym Full Names: cystatin A (stefin A)
• NCBI Official Symbol: CSTA
• NCBI Official Synonym Symbols: AREI; STF1; STFA
• NCBI Protein Information: cystatin-A; cystatin AS
• UniProt Protein Name: Cystatin-A
• Protein Family: Cystatin
• UniProt Gene Name: CSTA
• UniProt Synonym Gene Names: STF1; STFA
• UniProt Entry Name: CYTA_HUMAN

NCBI Description

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

CSTA: This is an intracellular thiol proteinase inhibitor. Has an important role in desmosome-mediated cell-cell adhesion in the lower levels of the epidermis. Defects in CSTA are the cause of ichthyosis exfoliative autosomal recessive ichthyosis bullosa of Siemens-like (AREI). A form of congenital exfoliative ichthyosis, sharing some features with ichthyosis bullosa of Siemens and annular epidermolytic ichthyosis. AREI presents shortly after birth as dry, scaly skin over most of the body with coarse peeling of non- erythematous skin on the palms and soles, which is exacerbated by excessive moisture and minor trauma. Electron microscopy analysis of skin biopsies, reveals mostly normal-appearing upper layers of the epidermis, but prominent intercellular edema of the basal and suprabasal cell layers with aggregates of tonofilaments in the basal keratinocytes. Belongs to the cystatin family.

Chromosomal Location of Human Ortholog: 3q21

Cellular Component: nucleoplasm; extracellular space; cytoplasm; cornified envelope; nucleus

Molecular Function: protein binding, bridging; protease binding; structural molecule activity; cysteine protease inhibitor activity

Biological Process: negative regulation of proteolysis; keratinocyte differentiation; cell-cell adhesion; negative regulation of peptidase activity; peptide cross-linking

Disease: Exfoliative Ichthyosis, Autosomal Recessive, Ichthyosis Bullosa Of Siemens-like