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Immunohistochemistry (IHC) ((0.03ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NODAL Antibody | anti-NODAL antibody

Goat anti-NODAL Antibody

Gene Names
NODAL; HTX5
Reactivity
Rat
Expected from sequence similarity: Human, Mouse
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
NODAL; Goat anti-NODAL Antibody; nodal growth differentiation factor; HTX5; anti-NODAL antibody
Ordering
For Research Use Only!
Reactivity
Rat
Expected from sequence similarity: Human, Mouse
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence Length
347
Applicable Applications for anti-NODAL antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Pep-ELISA: antibody detection limit dilution 1:64000.
WB: Approx 40kDa band observed in Rat Brain lysates. Recommended concentration: 0.01-0.1ug/ml.
Immunogen Sequence
Peptide with sequence DRSQLCRKVKFQ, from the internal region of the protein sequence according to NP_060525.2.
Peptide Sequence + C
DRSQLCRKVKFQ
Epitope
Internal Region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

((0.03ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Immunohistochemistry (IHC) ((0.03ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40.5kDa
NCBI Official Full Name
nodal
NCBI Official Synonym Full Names
nodal growth differentiation factor
NCBI Official Symbol
NODAL
NCBI Official Synonym Symbols
HTX5
NCBI Protein Information
nodal homolog
UniProt Protein Name
Nodal homolog
Protein Family
UniProt Gene Name
NODAL

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which regulates early embryonic development. This protein is required for maintenance of human embryonic stem cell pluripotency and may play a role in human placental development. Mutations in this gene are associated with heterotaxy, a condition characterized by random orientation of visceral organs with respect to the left-right axis. [provided by RefSeq, Aug 2016]

Uniprot Description

Essential for mesoderm formation and axial patterning during embryonic development.

Research Articles on NODAL

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Product Notes

The NODAL nodal (Catalog #AAA423726) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-NODAL Antibody reacts with Rat Expected from sequence similarity: Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NODAL can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Pep-ELISA: antibody detection limit dilution 1:64000. WB: Approx 40kDa band observed in Rat Brain lysates. Recommended concentration: 0.01-0.1ug/ml. Researchers should empirically determine the suitability of the NODAL nodal for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NODAL, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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